Asahina K, Suzuki K, Hibiya T, Tamaoki B
College of Agriculture and Veterinary Medicine, Nihon University, Tokyo, Japan.
Gen Comp Endocrinol. 1989 Jun;74(3):385-91. doi: 10.1016/s0016-6480(89)80035-8.
The in vitro steroid metabolism in the seminal vesicles of the brackish water goby (urohaze-goby, Glossogobius olivaceus) was studied using males in the breeding season. The moderate activity of delta 5-3 beta-hydroxysteroid dehydrogenase was histochemically detected only in the epithelial cells of the organ, though these cells have the characteristics of secretory cells ultrastructurally. Cell-free homogenates (800 g supernatant fluid) of the whole tissue were aerobically incubated with 14C-labeled pregnenolone, progesterone, 17 alpha-hydroxyprogesterone, androstenedione, dehydroepiandrosterone, or testosterone in the presence of NAD+ or NADPH. Pregnenolone and dehydroepiandrosterone were converted to progesterone and androstenedione, respectively. Progesterone was transformed to 5 alpha-pregnane-3,20-dione (main product) and 17 alpha-hydroxyprogesterone. 17 alpha-Hydroxyprogesterone was metabolized into androstenedione (main product) and 17 alpha-hydroxy-5 alpha-pregnane-3,20-dione. From androstenedione, 5 alpha-androstane-3,17-dione (main product) and epiandrosterone were obtained. Testosterone was transformed to 5 alpha-dihydrotestosterone, 5 alpha-androstane-3 beta, 17 beta-diol, 5 alpha-androstane-3,17-dione, and androstenedione. These results indicate that the steroid metabolic patterns in the seminal vesicles of G. olivaceus are closely resembled to those in the testes.
利用繁殖季节的雄性半咸水虾虎鱼（乌氏虾虎鱼，Glossogobius olivaceus）研究了其精囊中的体外类固醇代谢。组织化学检测发现，仅在该器官的上皮细胞中检测到δ5-3β-羟基类固醇脱氢酶的适度活性，不过这些细胞在超微结构上具有分泌细胞的特征。将整个组织的无细胞匀浆（800g上清液）与14C标记的孕烯醇酮、孕酮、17α-羟基孕酮、雄烯二酮、脱氢表雄酮或睾酮在NAD+或NADPH存在的条件下进行需氧孵育。孕烯醇酮和脱氢表雄酮分别转化为孕酮和雄烯二酮。孕酮转化为5α-孕烷-3,20-二酮（主要产物）和17α-羟基孕酮。17α-羟基孕酮代谢为雄烯二酮（主要产物）和17α-羟基-5α-孕烷-3,20-二酮。从雄烯二酮得到5α-雄烷-3,17-二酮（主要产物）和表雄酮。睾酮转化为5α-二氢睾酮、5α-雄烷-3β,17β-二醇、5α-雄烷-3,17-二酮和雄烯二酮。这些结果表明，乌氏虾虎鱼精囊中的类固醇代谢模式与睾丸中的类固醇代谢模式非常相似。
