a Department of Pharmacy , University "G. d'Annunzio" of Chieti-Pescar , Chieti , Italy.
b Department of Health Sciences , and.
J Enzyme Inhib Med Chem. 2016;31(sup3):110-116. doi: 10.1080/14756366.2016.1209496. Epub 2016 Jul 22.
We developed and validated an analytical method based on microextraction packed sorbent (MEPS) and high-performance liquid chromatography (HPLC) coupled to photodiode array (PDA) detector to simultaneously quantify multiple nonsteroidal anti-inflammatory drugs (NSAIDs) and fluoroquinolones (FLQs), which may provide as combination several adverse reactions in nephrology and neurology. The linearity range from LOQs (0.1 μg/mL) to 10 μg/mL, and LODs values were 0.03 μg/mL for both NSAIDs and FLQs. The validation was performed according to international guidelines and the accuracy was tested measuring the precision, intermediate precision and trueness. The drugs stability was tested under different storage conditions (+4 °C and -20 °C) and after three different cycles of freezing and thawing. The method can be a suitable tool to simultaneously detect a possible association of drugs in human biological samples and provide several potentialities for clinical applications, bioequivalence studies, pharmacodynamics and toxicodynamics of different pharmaceutical dosage forms showing NSAIDs and FLQs.
我们开发并验证了一种基于微萃取填充固相（MEPS）和高效液相色谱（HPLC）与光电二极管阵列（PDA）检测器相结合的分析方法，用于同时定量多种非甾体抗炎药（NSAIDs）和氟喹诺酮类药物（FLQs），这些药物可能会在肾病学和神经病学中引发多种不良反应。该方法的线性范围为从 LOQs（0.1μg/mL）到 10μg/mL，NSAIDs 和 FLQs 的检出限（LOD）值均为 0.03μg/mL。该验证符合国际指南进行，通过测量精密度、中间精密度和准确度来测试准确性。在不同的储存条件（+4°C 和 -20°C）下以及经过三次不同的冷冻和解冻循环后，测试了药物的稳定性。该方法可作为一种合适的工具，用于同时检测人类生物样本中药物的可能关联，并为临床应用、生物等效性研究、不同药物制剂的药效学和毒代动力学提供多种可能性，这些药物制剂均显示出 NSAIDs 和 FLQs 的作用。
