Kou Wei, Zhang Hua, Bibi Aisha, Ke Mufang, Han Jing, Xiong Jianliang, Su Rui, Liang Dapeng
State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University Changchun 130012 China.
School of Pharmaceutical Sciences, Peking University Beijing 100191 China.
RSC Adv. 2018 May 11;8(31):17293-17299. doi: 10.1039/c8ra01837e. eCollection 2018 May 9.
In this study, a facile method based on molecularly imprinted polymers (MIPs) combined with internal extractive electrospray ionization tandem mass spectrometry (iEESI-MS/MS) was developed for the quantitative analysis of fluoroquinolones (FQs) in environmental water samples. FQ molecules in water samples were captured by the MIPs, which was retained on a 0.22 μm syringe filter. Then, an electrospray solution selected as the elution solution was employed to extract the FQs from the MIPs, getting an eluate of FQs for mass spectrometric interrogation. Under the optimized experimental conditions, low limits of detection (LODs, 0.015-0.026 μg L), with relative standard deviations (RSDs) less than 8.81% ( = 6) were obtained. The present method also provides good recoveries (91.14-103.60%) with acceptable precision (RSDs < 6.18%) and have no serious matrix effects for environmental water samples. The experimental results demonstrated that MIPs-iEESI-MS/MS has advantages including easy use, high speed (less than 3 min per sample) and high sensitivity for the analysis of FQs in environmental water samples, showing potential application in environmental science and water safety control.
在本研究中，开发了一种基于分子印迹聚合物（MIPs）结合内萃取电喷雾电离串联质谱（iEESI-MS/MS）的简便方法，用于环境水样中氟喹诺酮类药物（FQs）的定量分析。水样中的FQ分子被MIPs捕获，MIPs保留在0.22μm的注射器滤膜上。然后，选用一种电喷雾溶液作为洗脱液，从MIPs中萃取FQs，得到用于质谱检测的FQs洗脱液。在优化的实验条件下，获得了低检测限（LODs，0.015 - 0.026μg/L），相对标准偏差（RSDs）小于8.81%（n = 6）。本方法对环境水样也具有良好的回收率（91.14 - 103.60%），精密度可接受（RSDs < 6.18%），且不存在严重的基质效应。实验结果表明，MIPs-iEESI-MS/MS在分析环境水样中的FQs时具有使用简便、速度快（每个样品少于3分钟）和灵敏度高的优点，在环境科学和水安全控制方面显示出潜在的应用价值。
