苯二氮䓬类药物对腺泡细胞和肌上皮细胞的影响。
Effects of Benzodiazepines on Acinar and Myoepithelial Cells.
作者信息
Mattioli Tatiana M F, Alanis Luciana R A, Sapelli Silvana da Silva, de Lima Antonio A S, de Noronha Lucia, Rosa Edvaldo A R, Althobaiti Yusuf S, Almalki Atiah H, Sari Youssef, Ignacio Sergio A, Johann Aline C B R, Gregio Ana M T
机构信息
Pharmacology and Experimental Pathology, School of Dentistry, Pontifical Catholic University of Paraná Curitiba, Brazil.
Program of Post-Graduation, School of Dentistry, Health and Bioscience School, Pontifical Catholic University of Paraná Curitiba, Brazil.
出版信息
Front Pharmacol. 2016 Jun 24;7:173. doi: 10.3389/fphar.2016.00173. eCollection 2016.
BACKGROUND
Benzodiazepines (BZDs), the most commonly prescribed psychotropic drugs with anxiolytic action, may cause hyposalivation. It has been previously shown that BZDs can cause hypertrophy and decrease the acini cell number. In this study, we investigated the effects of BZDs and pilocarpine on rat parotid glands, specifically on acinar, ductal, and myoepithelial cells.
METHODS
Ninety male Wistar rats were divided into nine groups. Control groups received a saline solution for 30 days (C30) and 60 days (C60), and pilocarpine (PILO) for 60 days. Experimental groups received lorazepam (L30) and midazolam (M30) for 30 days. Another group (LS60 or MS60) received lorazepam or midazolam for 30 days, respectively, and saline for additional 30 days. Finally, other groups (LP60 or MP60) received either lorazepam or midazolam for 30 days, respectively, and pilocarpine for additional 30 days. The expression of calponin in myoepithelial cells and the proliferating cell nuclear antigen (PCNA) in acinar and ductal cells were evaluated.
RESULTS
Animals treated with lorazepam showed an increase in the number of positive staining cells for calponin as compared to control animals (p < 0.05). Midazolam administered with pilocarpine (MP60) induced an increase in the proliferation of acinar and ductal cells and a decrease in the positive staining cells for calponin as compared to midazolam administered with saline (MS60).
CONCLUSION
We found that myoepithelial cells might be more sensitive to the effects of BZD than acinar and ductal cells in rat parotid glands.
背景
苯二氮䓬类药物（BZDs）是最常用的具有抗焦虑作用的精神药物，可能导致唾液分泌减少。先前的研究表明，BZDs可导致肥大并减少腺泡细胞数量。在本研究中，我们调查了BZDs和毛果芸香碱对大鼠腮腺的影响，特别是对腺泡、导管和肌上皮细胞的影响。
方法
将90只雄性Wistar大鼠分为9组。对照组接受生理盐水30天（C30）和60天（C60），以及毛果芸香碱（PILO）60天。实验组接受劳拉西泮（L30）和咪达唑仑（M30）30天。另一组（LS60或MS60）分别接受劳拉西泮或咪达唑仑30天，然后再接受生理盐水30天。最后，其他组（LP60或MP60）分别接受劳拉西泮或咪达唑仑30天，然后再接受毛果芸香碱30天。评估肌上皮细胞中钙调蛋白的表达以及腺泡和导管细胞中增殖细胞核抗原（PCNA）的表达。
结果
与对照动物相比，接受劳拉西泮治疗的动物中钙调蛋白阳性染色细胞数量增加（p < 0.05）。与接受生理盐水的咪达唑仑组（MS60）相比，联合毛果芸香碱的咪达唑仑组（MP60）诱导腺泡和导管细胞增殖增加，钙调蛋白阳性染色细胞减少。
结论
我们发现大鼠腮腺中的肌上皮细胞可能比腺泡和导管细胞对BZD的作用更敏感。
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