Artoni Pietro, Landi Silvia, Sato Sebastian Sulis, Luin Stefano, Ratto Gian Michele
NEST, Istituto Nanoscienze CNR and Scuola Normale Superiore, Pisa, 56127 Italy.
Biomed Opt Express. 2016 Mar 30;7(4):1604-13. doi: 10.1364/BOE.7.001604. eCollection 2016 Apr 1.
Two-photon excitation spectroscopy is a powerful technique for the characterization of the optical properties of genetically encoded and synthetic fluorescent molecules. Excitation spectroscopy requires tuning the wavelength of the Ti:sapphire laser while carefully monitoring the delivered power. To assist laser tuning and the control of delivered power, we developed an Arduino Due based tool for the automatic acquisition of high quality spectra. This tool is portable, fast, affordable and precise. It allowed studying the impact of scattering and of blood absorption on two-photon excitation light. In this way, we determined the wavelength-dependent deformation of excitation spectra occurring in deep tissues in vivo.
双光子激发光谱是一种用于表征基因编码和合成荧光分子光学性质的强大技术。激发光谱需要在仔细监测输出功率的同时调节钛宝石激光器的波长。为了辅助激光调谐和输出功率控制，我们开发了一种基于Arduino Due的工具，用于自动采集高质量光谱。该工具便携、快速、经济且精确。它有助于研究散射和血液吸收对双光子激发光的影响。通过这种方式，我们确定了体内深部组织中激发光谱随波长的变化。
