Li Hongmei, Park Han-A, Jonas Elizabeth A
Department of Internal Medicine (Endocrinology), School of Medicine, Yale University, New Haven, USA.
Department of Internal Medicine (Endocrinology), School of Medicine, Yale University, New Haven, USA; Department of Neurobiology, School of Medicine, Yale University, New Haven, USA.
Bio Protoc. 2014 Dec 5;4(23). doi: 10.21769/bioprotoc.1304.
This protocol comprises the entire process of fluorescent measurement of vesicle recycling using the probe SynaptopHluorin, a pH-dependent GFP variant whose fluorescence increases at the synapse upon vesicle release due to fluorescence quenching in acidic vesicles. This technique provides a genetic tool to monitor synaptic vesicle recycling in real time in cultured hippocampal neurons.