Janštová Žofia, Burkuš Ján, Kubandová Janka, Fabian Dušan, Koppel Juraj, Čikoš Štefan
Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4, 04001 Košice, Slovak Republic.
Gen Physiol Biophys. 2017 Jan;36(1):53-63. doi: 10.4149/gpb_2016019. Epub 2016 Jul 22.
The effect of maternal stress on blastocyst quality, with respect to maternal metabolic status, was investigated in this study. We exposed female mice with different amounts of body fat to restraint stress and examined their blastocyst quality. Blood concentrations of corticosterone, leptin, adiponectin, insulin and glucose were measured in these females. Significantly lower stress-induced corticosterone elevations were observed in females with high and low amounts of body fat, indicating that the stress response was altered in these females. The basal leptin concentrations were significantly higher in females with high amounts of body fat than in females with low amounts of body fat, and stress induced different responses in these two groups of females. Our results showed that maternal stress can significantly increase the proportion of blastocysts that contain dead (apoptotic) cells in females with high and medium amounts of body fat. In females with low amounts of body fat, the proportion of blastocysts containing dead (apoptotic) cells was already increased before the stress exposure, and application of stress did not significantly change this parameter. Our results showed that the effects of maternal stress on early embryos can depend on the actual physiological status of the maternal organism exposed to stress.
本研究调查了母体应激对囊胚质量的影响，以及母体代谢状态与该影响之间的关系。我们使不同体脂量的雌性小鼠遭受束缚应激，并检测它们的囊胚质量。测定了这些雌性小鼠血液中的皮质酮、瘦素、脂联素、胰岛素和葡萄糖浓度。体脂量高和低的雌性小鼠应激诱导的皮质酮升高显著较低，表明这些雌性小鼠的应激反应发生了改变。体脂量高的雌性小鼠基础瘦素浓度显著高于体脂量低的雌性小鼠，应激在这两组雌性小鼠中诱导了不同的反应。我们的结果表明，母体应激可显著增加体脂量高和中等的雌性小鼠中含有死亡（凋亡）细胞的囊胚比例。在体脂量低的雌性小鼠中，含有死亡（凋亡）细胞的囊胚比例在应激暴露前就已增加，施加应激并未显著改变这一参数。我们的结果表明，母体应激对早期胚胎的影响可能取决于遭受应激的母体生物体的实际生理状态。
