Lee Seulah, Moon Eunjung, Choi Sang Un, Kim Ki Hyun
School of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Korea.
Charmzone R&D Center, Charmzone Co., Ltd., 318 Yeongdong-daero, Gangnam-gu, Seoul, 06177, Korea.
Chem Biodivers. 2016 Oct;13(10):1391-1396. doi: 10.1002/cbdv.201600083.
A new sesquilignan, euonymolin A (1), and six known lignans, (-)-de-O-methylmagnolin (2), (+)-de-O-methylepimagnolin A (3), (+)-syringaresinol (4), (+)-pinoresinol (5), (+)-medioresinol (6), and (+)-lariciresinol 4'-O-β-d-glucopyranoside (7), were isolated from the twigs of Euonymus alatus (Thunb.) Siebold (Celastraceae). The structures of the isolated compounds were elucidated based on spectroscopic analyses, including extensive 1D- and 2D-NMR techniques, HR-MS analysis and circular dichroism (CD) data, and the literature data. All of the isolated compounds were evaluated for antiproliferative activity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines and inhibition of nitric oxide (NO) production in a lipopolysaccharide (LPS)-activated BV2 cell line. All compounds showed cytotoxicity against the SK-MEL-2 cell line with IC values of 23.24 - 48.14 μm and inhibited NO production in LPS-activated BV-2 cells with IC values of 6.75 - 23.53 μm.
从卫矛（卫矛科）的嫩枝中分离出一种新的倍半木脂素，卫矛醇A（1），以及六种已知的木脂素，（-）-去-O-甲基木兰脂素（2），（+）-去-O-甲基表木兰脂素A（3），（+）-紫丁香树脂醇（4），（+）-松脂醇（5），（+）-中树脂醇（6）和（+）-落叶松脂醇4'-O-β-D-吡喃葡萄糖苷（7）。基于光谱分析，包括广泛的一维和二维核磁共振技术、高分辨质谱分析和圆二色性（CD）数据以及文献数据，阐明了分离得到的化合物的结构。对所有分离得到的化合物进行了针对A549、SK-OV-3、SK-MEL-2和HCT-15细胞系的抗增殖活性评估，以及对脂多糖（LPS）激活的BV2细胞系中一氧化氮（NO）产生的抑制作用评估。所有化合物对SK-MEL-2细胞系均表现出细胞毒性，IC值为23.24 - 48.14μm，并且对LPS激活的BV-2细胞中NO的产生具有抑制作用，IC值为6.75 - 23.53μm。
