Lee Sung-Ju, Jang Seon-A, Kim Seong Cheol, Gu Dong Ryun, Yang Hyun, Ryuk Jin Ah, Ha Hyunil
KM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Daejeon 34054, Republic of Korea.
Future Technology Research Center, KT&G Corporation, 30, Gajeong-ro, Yuseong-gu, Daejeon 34128, Republic of Korea.
Nutrients. 2023 Sep 15;15(18):3996. doi: 10.3390/nu15183996.
(Thunb.) Siebold, a traditional medicinal plant, has been used in China and several other Asian countries to address a variety of health concerns. The extensive research conducted on is driven by its diverse pharmacological applications. However, its biological effects on osteoclastogenesis and osteoporosis have not been previously studied. In this research, we investigated the impact of an ethanolic extract of (EEEA) on osteoclast differentiation and function as well as estrogen deficiency-induced bone loss. We found that EEEA inhibits osteoclast differentiation by downregulating the expression of the receptor activator of nuclear factor-κB ligand (RANKL) in osteoclast-supporting cells and by directly impeding RANKL-mediated signaling pathways for osteoclastogenesis in precursor cells. In addition, EEEA inhibited the bone-resorptive function of mature osteoclasts in vitro. Furthermore, oral administration of EEEA significantly alleviated bone loss in an ovariectomy-induced osteoporosis mouse model. Additionally, we identified phytochemicals in EEEA that have suppressive effects on osteoclast differentiation and bone loss. Collectively, these results suggest that EEEA holds potential as a biotherapeutic candidate for anti-postmenopausal osteoporosis.
（Thunb.）Siebold是一种传统药用植物，在中国和其他几个亚洲国家已被用于解决各种健康问题。对其进行广泛研究的动力来自于其多样的药理应用。然而，其对破骨细胞生成和骨质疏松症的生物学效应此前尚未得到研究。在本研究中，我们调查了（Thunb.）Siebold乙醇提取物（EEEA）对破骨细胞分化和功能以及雌激素缺乏引起的骨质流失的影响。我们发现，EEEA通过下调破骨细胞支持细胞中核因子κB受体活化因子配体（RANKL）的表达，并直接阻碍前体细胞中RANKL介导的破骨细胞生成信号通路，来抑制破骨细胞分化。此外，EEEA在体外抑制成熟破骨细胞的骨吸收功能。此外，口服EEEA可显著减轻卵巢切除诱导的骨质疏松小鼠模型中的骨质流失。此外，我们在EEEA中鉴定出了对破骨细胞分化和骨质流失具有抑制作用的植物化学物质。总体而言，这些结果表明，EEEA有望成为抗绝经后骨质疏松症的生物治疗候选药物。
