Sobeh Mansour, Braun Markus Santhosh, Krstin Sonja, Youssef Fadia S, Ashour Mohamed L, Wink Michael
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, DE-69120, Heidelberg, Germany.
Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University Abbassia, 11566, Cairo, Egypt.
Chem Biodivers. 2016 Nov;13(11):1537-1550. doi: 10.1002/cbdv.201600089. Epub 2016 Nov 2.
The essential oil compositions of the leaves of three related Myrtaceae species, namely Syzygium aqueum, Syzygium samarangense and Eugenia uniflora, were investigated using GLC/MS and GLC/FID. Altogether, 125 compounds were identified: α-Selinene (13.85%), β-caryophyllene (12.72%) and β-selinene constitute the most abundant constituents in S. aqueum. Germacrene D (21.62%) represents the major compound in S. samarangense whereas in E. uniflora, spathulenol (15.80%) represents the predominant component. Multivariate chemometric analyses were used to discriminate the essential oils using hierarchical cluster analysis (HCA) and principal component analysis (PCA) based on the chromatographic results. The antimicrobial activity of the popularly used E. uniflora essential oil was assessed using broth microdilution method against six Gram-positive, three Gram-negative bacteria and two fungi. The oil showed moderate antimicrobial activity against Bacillus licheniformis exhibiting MIC and MMC of 0.63 mg/ml. The cytotoxic activity of E. uniflora essential oil was investigated against Trypanosoma brucei brucei (T. b. brucei) and MCF-7 cancer cell line using MTT assay. It showed moderate activity against MCF-7 cells with an IC value of 76.40 μg/ml. On the other hand, T. brucei was highly susceptible to E. uniflora essential oil with IC of 11.20 μg/ml, and a selectivity index of 6.82.
采用气相色谱/质谱联用（GLC/MS）和气相色谱/氢火焰离子化检测器联用（GLC/FID）技术，对桃金娘科三种近缘植物水蒲桃（Syzygium aqueum）、洋蒲桃（Syzygium samarangense）和红果仔（Eugenia uniflora）叶片的精油成分进行了研究。共鉴定出125种化合物：水蒲桃中含量最丰富的成分是α-芹子烯（13.85%）、β-石竹烯（12.72%）和β-芹子烯。吉马烯D（21.62%）是洋蒲桃中的主要化合物，而在红果仔中，匙叶桉油烯醇（15.80%）是主要成分。基于色谱结果，采用多元化学计量学分析方法，通过层次聚类分析（HCA）和主成分分析（PCA）对精油进行鉴别。采用肉汤微量稀释法，评估了常用的红果仔精油对六种革兰氏阳性菌、三种革兰氏阴性菌和两种真菌的抗菌活性。该精油对licheniformis芽孢杆菌表现出中等抗菌活性，最低抑菌浓度（MIC）和最低杀菌浓度（MMC）为0.63mg/ml。采用MTT法研究了红果仔精油对布氏布氏锥虫（Trypanosoma brucei brucei）和MCF-7癌细胞系的细胞毒性活性。它对MCF-7细胞表现出中等活性，IC值为76.40μg/ml。另一方面，布氏锥虫对红果仔精油高度敏感，IC为11.20μg/ml，选择性指数为6.82。
