Lin Chao, Zhao Guo-Chao, Xu Ya-Dong, Wang Dan-Song, Jin Da-Yong, Ji Yuan, Lou Wen-Hui, Wu Wen-Chuan
Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai, China.
Oncotarget. 2016 Sep 13;7(37):60657-60664. doi: 10.18632/oncotarget.10630.
αTubulin, the essential orchestrator of cytoskeletal protein polymers, critical for cell growth and division, motility, signaling development and maintenance of cell shape, plays vital roles in the oncogenesis and progression of various types of cancer, but its role in prognosis of pancreatic cancer patients remains unknown. The aim of this study was to investigate its prognostic value in patients with pancreatic cancer after surgical resection.
αTubulin expression in pancreatic cancer was significantly associated with N classification (p = 0.013) and TNM stage (p = 0.025). Increased expression of αTubulin in tumoral tissue was associated with decreased overall survival rate (p = 0.002). Multivariate Cox regression analysis suggested that αTubulin expression was an independent prognostic indicator for pancreatic cancer except for T and N classification (p = 0.002). Using multivariate analysis, αTubulin expression, CA19-9, and N classification were selected to generate the nomogram to predict the 1-year and 3-year overall survival. The c-index of this model was 0.692. The calibration curve for probability of survival showed good agreement between prediction by nomogram and actual observation.
αTubulin expression was evaluated by tissue microarrays from 124 pancreatic cancer patients and statistically assessed for correlations with the clinical profiles and the prognosis of the patients with pancreatic cancer. The prognostic nomogram was designed to predict 1-year and 3-year overall survival probability.
αTubulin expression might be an independent prognostic factor for pancreatic cancer after surgical resection and could potentially be a high-priority therapeutic target. Incorporating αTubulin expression into CA19-9 and N classification can provide a good prognostic model.
α微管蛋白是细胞骨架蛋白聚合物的重要协调者，对细胞生长、分裂、运动、信号传导、发育及细胞形态维持至关重要，在各类癌症的发生和发展中发挥着重要作用，但其在胰腺癌患者预后中的作用尚不清楚。本研究旨在探讨其在胰腺癌手术切除患者中的预后价值。
胰腺癌中α微管蛋白的表达与N分期（p = 0.013）和TNM分期（p = 0.025）显著相关。肿瘤组织中α微管蛋白表达增加与总生存率降低相关（p = 0.002）。多变量Cox回归分析表明，除T和N分期外，α微管蛋白表达是胰腺癌的独立预后指标（p = 0.002）。通过多变量分析，选择α微管蛋白表达、CA19-9和N分期来生成列线图，以预测1年和3年总生存率。该模型的c指数为0.692。生存概率校准曲线显示列线图预测与实际观察结果之间具有良好的一致性。
通过组织芯片评估124例胰腺癌患者的α微管蛋白表达，并对其与临床特征及胰腺癌患者预后的相关性进行统计学评估。设计预后列线图以预测1年和3年总生存概率。
α微管蛋白表达可能是胰腺癌手术切除后的独立预后因素，并且可能是一个高度优先的治疗靶点。将α微管蛋白表达纳入CA19-9和N分期可提供一个良好的预后模型。
