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完整的叶绿体基因组序列与. 的系统发育分析

Complete Chloroplast Genome Sequence and Phylogenetic Analysis of .

机构信息

Artemisinin Research Center, Institute of Chinese Materia Medical, China Academy of Chinese Medical Sciences, Beijing 100700, China.

College of Agricultural (College of Tree Peony), Henan University of Science and Technology, Luoyang 471023, China.

出版信息

Molecules. 2018 Sep 21;23(10):2426. doi: 10.3390/molecules23102426.

Abstract

We sequenced and analyzed the complete chloroplast genome of (family Asteraceae), a Chinese herb used medicinally to relieve coughs and reduce sputum. The chloroplast genome was 152,992 bp in size, and harbored a pair of inverted repeat regions (IRa and IRb, each 24,850 bp) divided into a large single-copy (LSC, 84,698 bp) and a small single-copy (SSC, 18,250 bp) region. Our annotation revealed that the chloroplast genome contained 115 genes, including 81 protein-coding genes, 4 ribosomal RNA genes, and 30 transfer RNA genes. In addition, 70 simple sequence repeats (SSRs) were detected in the chloroplast genome, including mononucleotides (36), dinucleotides (1), trinucleotides (23), tetranucleotides (1), pentanucleotides (8), and hexanucleotides (1). Comparative chloroplast genome analysis of three species indicated that a higher similarity was preserved in the IR regions than in the LSC and SSC regions, and that the differences in the degree of preservation were slighter between and than between and . Phylogenetic analysis revealed that was more closely related to than to . Our findings offer valuable information for future research on species identification and selective breeding.

摘要

我们对一种中国草药(菊科)的完整叶绿体基因组进行了测序和分析,该草药用于止咳化痰。叶绿体基因组大小为 152992bp,包含一对反向重复区(IRa 和 IRb,各 24850bp),分为一个大单拷贝区(LSC,84698bp)和一个小单拷贝区(SSC,18250bp)。我们的注释表明,叶绿体基因组包含 115 个基因,包括 81 个蛋白编码基因、4 个核糖体 RNA 基因和 30 个转移 RNA 基因。此外,在叶绿体基因组中检测到 70 个简单序列重复(SSR),包括单核苷酸(36)、二核苷酸(1)、三核苷酸(23)、四核苷酸(1)、五核苷酸(8)和六核苷酸(1)。对三个物种的叶绿体基因组比较分析表明,IR 区的相似度高于 LSC 和 SSC 区,而在 和 之间的保存程度差异比在 和 之间的差异更小。系统发育分析表明,与 相比, 与 的亲缘关系更密切。我们的研究结果为今后对 物种鉴定和选择性育种的研究提供了有价值的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f04/6222381/281f887161ac/molecules-23-02426-g001.jpg

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