Levin B E, Sullivan A C
Neurology Service, Veterans Administration Medical Centre, East Orange, New Jersey 07019.
Int J Obes. 1989;13(2):235-46.
Half of the 3-month-old male Sprague-Dawley rats fed a diet enriched in calories, sucrose and fat (CM diet) for 3 months developed diet-induced obesity (DIO) while the remainder were diet resistant (DR), gaining the same amount of weight as chow-fed controls. DIO was associated with basal hyperinsulinemia and 196 percent heavier retroperitoneal fat pads than DR and chow-fed rats. Intravenous glucose infusion (lg/kg) also produced greater insulin release in DIO rats associated with a 25 percent reduction in glucose disposal rates; areas under the insulin curve were 273 and 51 percent higher than chow-fed and DR rats, respectively. DR rats, on the other hand, showed normal glucose disposal rates but increased insulin release to a glucose load (148 percent greater area under the curve than chow-fed rats). Glucose infusion produced activation of the sympathetic nervous system with release of norepinephrine (NE) into the plasma in chow-fed and DIO but not DR rats which had 40-50 percent lower areas under the NE curve. Also, the areas under the NE curve correlated with body weight gain (r = 0.632; P = 0.040) and retroperitoneal fat pad weight (r = 0.707; P = 0.030) in DR and DIO rats suggesting that glucose-induced sympathetic activation was in some way related to an underlying mechanism of body weight regulation in Sprague-Dawley rats.
给3个月大的雄性斯普拉格-道利大鼠喂食富含热量、蔗糖和脂肪的饮食（CM饮食）3个月，其中一半大鼠出现饮食诱导性肥胖（DIO），而其余大鼠对饮食有抗性（DR），体重增加量与喂食普通饲料的对照组相同。DIO与基础高胰岛素血症相关，其腹膜后脂肪垫比DR大鼠和喂食普通饲料的大鼠重196%。静脉注射葡萄糖（1g/kg）也使DIO大鼠产生更大的胰岛素释放，同时葡萄糖处置率降低25%；胰岛素曲线下面积分别比喂食普通饲料的大鼠和DR大鼠高273%和51%。另一方面，DR大鼠的葡萄糖处置率正常，但对葡萄糖负荷的胰岛素释放增加（曲线下面积比喂食普通饲料的大鼠大148%）。葡萄糖输注使喂食普通饲料的大鼠和DIO大鼠的交感神经系统激活，血浆中去甲肾上腺素（NE）释放，但DR大鼠没有，DR大鼠的NE曲线下面积低40 - 50%。此外，DR大鼠和DIO大鼠的NE曲线下面积与体重增加（r = 0.632；P = 0.040）和腹膜后脂肪垫重量（r = 0.707；P = 0.030）相关，这表明葡萄糖诱导的交感神经激活在某种程度上与斯普拉格-道利大鼠体重调节的潜在机制有关。
