Azemard Clara, Menager Matthieu, Vieillescazes Catherine
Equipe Ingénierie de la Restauration des Patrimoines Naturel et Culturel-IMBE UMR 7263 CNRS-IRD 237, Université d'Avignon et des Pays de Vaucluse, 84000, Avignon, France.
Anal Bioanal Chem. 2016 Sep;408(24):6599-612. doi: 10.1007/s00216-016-9772-9. Epub 2016 Jul 23.
The three principal types of molecules composing diterpenic resins are the abietanes, pimaranes and labdanes. The study of their fragmentation was performed by gas chromatography coupled to an ion trap mass spectrometer, on standards and resins used in paint varnishes: colophony and sandarac. We found that the general fragmentation pattern was mostly governed by the location of the double bonds on the different cycles and the presence of functional groups, and not by the nature of the C13 group in the case of abietanes and pimaranes. As for the labdanes, the loss of their alkyl chain is very specific. This study develops an analytical strategy using tandem mass spectrometry (MS/MS) experiments to validate the proposed mechanisms of fragmentation and to find the ions of interest for the identification of diterpenic molecules. Graphical Abstract Analysis of diterpenic compounds by GC-MS/MS.
构成二萜树脂的三种主要分子类型是枞酸型、海松烷型和半日花烷型。通过气相色谱与离子阱质谱联用，对用于油漆清漆的标准品和树脂（松香和山达脂）进行了它们的裂解研究。我们发现，一般的裂解模式主要受不同环上双键的位置和官能团的存在影响，而在枞酸型和海松烷型的情况下，不受C13基团性质的影响。至于半日花烷型，其烷基链的丢失非常特殊。本研究开发了一种分析策略，使用串联质谱（MS/MS）实验来验证所提出的裂解机制，并找到用于鉴定二萜分子的目标离子。图形摘要：通过GC-MS/MS分析二萜化合物。
