Key Laboratory of Applied Surface and Colloid Chemistry of Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China.
Key Laboratory of Applied Surface and Colloid Chemistry of Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Jan 5;170:198-205. doi: 10.1016/j.saa.2016.07.026. Epub 2016 Jul 16.
An imidazolium-modified pyrene derivative, IPy, was used for ratiometric detection of heparin, and its complex with heparin was used for reversed ratiometric detection of protamine in both aqueous solution and serum samples. The cationic fluorescent probe could interact with anionic heparin via electrostatic interaction to bring about blue-to-green fluorescence changes as monomer emission significantly decreases and excimer increases. The binary combination of IPy and heparin could be further used for green-to-blue detection of protamine since heparin prefers to bind to protamine instead of the probe due to its stronger affinity with protamine. The cationic probe shows high sensitivity to heparin with a low detection limit of 8.5nM (153ng/mL) and its combination with heparin displays high sensitivity to protamine with a detection limit as low as 15.4nM (107.8ng/mL) according to the 3σ IUPAC criteria. Moreover, both sensing processes are fast and can be performed in serum solutions, indicating possibility for practical applications.
一种咪唑基改性的芘衍生物 IPy 被用于肝素的比率检测，其与肝素的复合物被用于在水溶液和血清样品中对鱼精蛋白进行反向比率检测。阳离子荧光探针可以通过静电相互作用与阴离子肝素相互作用，从而导致单体发射显著减少，而激基缔合物增加，从而发生蓝到绿的荧光变化。由于肝素与鱼精蛋白的亲和力更强，因此 IPy 和肝素的二元混合物可以进一步用于绿色到蓝色检测鱼精蛋白。由于其与肝素的亲和力更强，该阳离子探针对肝素具有很高的灵敏度，检测限低至 8.5nM（153ng/mL），其与肝素的组合对鱼精蛋白也具有很高的灵敏度，检测限低至 15.4nM（107.8ng/mL），根据 3σ IUPAC 标准。此外，两种感应过程都很快，可以在血清溶液中进行，表明其具有实际应用的可能性。
