Bioorganic Chemistry Laboratory, Center for Design and Applications of Molecular Catalysts, Department of Chemistry and Chemical Engineering, Inha University , 253 Yonghyeon-dong, Nam-gu, Incheon 402-751, Republic of Korea.
Anal Chem. 2014 Jul 1;86(13):6580-6. doi: 10.1021/ac501089m. Epub 2014 Jun 18.
A peptidyl fluorescent chemosensor for heparin was synthesized by conjugating a pyrene fluorophore with the heparin-binding peptide. The fluorescent chemosensor (Py12; pyrene-RKRLQVQLSIRT) showed a highly sensitive ratiometric response to nanomolar concentrations of heparin in aqueous solutions at physiological pH by increasing excimer emission intensity at 500 nm with a concomitant decrease in monomer emission intensity at 400 nm. Py12 showed a sensitive ratiometric response to heparin over a wide pH range (1.5 ≤ pH ≤ 11.5) and exhibited high selectivity for heparin compared to other biological competitors, such as hyaluronic acid and chondroitin sulfate. Py12 sensitively and ratiometrically detected nanomolar concentrations of heparin in biologically relevant samples containing human serum and human plasma, respectively. The detection limit of Py12 was 34 pM (R(2) = 0.997) for heparin in an aqueous buffer solutions containing 5% human serum and 33 pM (R(2) = 0.994) for heparin in aqueous buffer solutions containing 5% human plasma. Py12 had sufficient sensitivity and selectivity for ratiometrically detecting a nanomolar concentration of heparin, indicating that the peptide-base chemosensor provides a potential tool for monitoring heparin levels in clinical plasma samples.
一种肽基荧光化学传感器通过将荧光团与肝素结合肽缀合而合成。荧光化学传感器（Py12；pyrene-RKRLQVQLSIRT）在生理 pH 值的水溶液中对纳米级浓度的肝素表现出高度灵敏的比率响应，通过增加 500nm 处的激基缔合物发射强度，同时降低 400nm 处的单体发射强度。Py12 在较宽的 pH 范围内（1.5 ≤ pH ≤ 11.5）对肝素表现出灵敏的比率响应，与其他生物竞争物（如透明质酸和硫酸软骨素）相比，对肝素具有高选择性。Py12 分别在含有人血清和人血浆的生物相关样品中灵敏且比率地检测出纳米级浓度的肝素。在含有 5%人血清的水溶液缓冲液中，Py12 对肝素的检测限为 34 pM（R² = 0.997），在含有 5%人血浆的水溶液缓冲液中，Py12 对肝素的检测限为 33 pM（R² = 0.994）。Py12 对肝素的纳米级浓度具有足够的灵敏度和选择性，表明基于肽的化学传感器为监测临床血浆样品中的肝素水平提供了一种潜在工具。
