Hakoda Masaru
Div. of Environmental Engineering Science, Faculty of Science and Technology, Gunma University, Japan.
Biotechnol Prog. 2016 Sep;32(5):1292-1300. doi: 10.1002/btpr.2330. Epub 2016 Aug 3.
Our previous studies revealed that the dielectrophoresis method is effective for separating cells having different dielectric properties. The purpose of this study was to evaluate the separation characteristics of two kinds of cells by direct current (DC) voltage offset/alternating current (AC) voltage using an insulating porous membrane dielectrophoretic separator. The separation device gives dielectrophoretic (DEP) force and electrophoretic (EP) force to dispersed particles by applying the DC-offset AC voltage. This device separates cells of different DEP properties by adopting a structure in which only the parallel plate electrodes and the insulating porous membrane are disposed in the flow path through which the cell-suspension flows. The difference in the retention ratios of electrically homogeneous 4.5 μm or 20.0 μm diameter standard particles was a maximum of 82 points. Furthermore, the influences of the AC voltage or offset voltage on the retention ratios of mouse hybridoma 3-2H3 cells and horse red blood cells (HRBC) were investigated. The difference in the retention ratio of the two kinds of cells was a maximum of 56 points. The separation efficiency of this device is expected to be improved by changing the device shape, number of pores, and pore placement. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1292-1300, 2016.
我们之前的研究表明，介电泳方法对于分离具有不同介电特性的细胞是有效的。本研究的目的是使用绝缘多孔膜介电泳分离器，通过直流（DC）电压偏移/交流（AC）电压来评估两种细胞的分离特性。该分离装置通过施加直流偏移交流电压，给分散的颗粒施加介电泳（DEP）力和电泳（EP）力。此装置采用一种结构，在细胞悬浮液流动的流路中仅设置平行板电极和绝缘多孔膜，从而分离具有不同DEP特性的细胞。直径为4.5μm或20.0μm的电均匀标准颗粒的保留率差异最大为82个百分点。此外，还研究了交流电压或偏移电压对小鼠杂交瘤3 - 2H3细胞和马红细胞（HRBC）保留率的影响。两种细胞的保留率差异最大为56个百分点。预计通过改变装置形状、孔的数量和孔的布局可提高该装置的分离效率。© 2016美国化学工程师学会生物技术进展，32:1292 - 1300，2016年。
