Tyler T A, Genzale J A
Herbert V. Shuster, Inc., Quincy, MA 02171.
J Assoc Off Anal Chem. 1989 May-Jun;72(3):442-4.
A rapid method has been developed for the determination of miconazole nitrate in creams and suppositories. The sample is dissolved in ethanol, diluted in acetonitrile-water (1 + 1), and injected onto a C18 column. The mobile phase consists of 55% acetonitrile, a triethylammonium phosphate buffer, and an ion-pairing agent. The total run time is less than 4 min, and the active ingredient is determined using absorbance detection at 214 nm. The mean recovery of miconazole from spiked placebo samples was 99.7 +/- 0.7% for the cream samples at the 2% level and 98.8 +/- 0.3% for the suppository samples at the 4% level.
已开发出一种快速测定乳膏剂和栓剂中硝酸咪康唑的方法。样品溶于乙醇，用乙腈 - 水（1 + 1）稀释后注入C18柱。流动相由55%乙腈、磷酸三乙铵缓冲液和离子对试剂组成。总运行时间少于4分钟，使用214nm处的吸光度检测来测定活性成分。对于加标安慰剂样品，乳膏剂样品在2%水平时咪康唑的平均回收率为99.7±0.7%，栓剂样品在4%水平时为98.8±0.3%。
