Welling P L, van Duyvenbode M C, Kaandorp B H
J Assoc Off Anal Chem. 1985 Jul-Aug;68(4):650-2.
A simple liquid chromatographic procedure is described for determination of the preservative dehydroacetic acid (DHA) in wine. No cleanup procedure is necessary; the sample is injected onto an NH2 column without further pretreatment. The mobile phase is a 9 + 1 mixture of acetonitrile-sodium acetate buffer adjusted to pH 4 with acetic acid. Total run time is less than 10 min. Dehydroacetic acid is determined by using the absorbance at 307 nm; the detection limit is estimated to be 0.2 ppm. The method may also be suitable for detecting DHA in a variety of foodstuffs in low concentrations.
本文描述了一种简单的液相色谱法，用于测定葡萄酒中的防腐剂脱氢乙酸（DHA）。无需净化步骤；样品无需进一步预处理即可直接注入氨基柱。流动相是乙腈 - 用乙酸调节至pH 4的醋酸钠缓冲液的9 + 1混合物。总运行时间少于10分钟。通过在307 nm处的吸光度测定脱氢乙酸；检测限估计为0.2 ppm。该方法也可能适用于检测低浓度各种食品中的DHA。
