Guo Long, Duan Li, Dou Li-Li, Liu Le-Le, Yang Hua, Liu E-Hu, Li Ping
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
J Pharm Biomed Anal. 2016 Sep 10;129:320-331. doi: 10.1016/j.jpba.2016.07.023. Epub 2016 Jul 16.
Selection of suitable labeled constituents is vital for the quality standardization of herbal medicines (HMs). However, discovery of labeled constituents that can account for the whole efficacy of original HMs is a challenging issue. Taking tanshinones extract (TE) as an example, a strategy to establish reasonable quality control method using effective compounds combination (ECC) as labeled constituents was proposed. The strategy consists of three core steps, including chemical profiling of TE, discovery and in vivo process research of ECC, and quality standardization based on ECC. Using this strategy, a combination of four tanshinones (tanshinones IIA, cryptotanshinone, tanshinone I and dihydrotanshinone I), which was as effective as TE in cell models and in a rat model of myocardial ischemia-reperfusion, was identified as ECC in TE. Furthermore, quality standardization of TE was established based on proposed ECC using single standard for determination of multiple components (SSDMC) method. In conclusion, the presently developed ECC-based approach not only offers new insight into the understanding of the holistic effects of HMs, but also provides efficacy-associated labeled constituents for quality control of botanicals.
选择合适的标记成分对于草药的质量标准化至关重要。然而，发现能够解释原生草药全部功效的标记成分是一个具有挑战性的问题。以丹参酮提取物（TE）为例，提出了一种以有效化合物组合（ECC）作为标记成分来建立合理质量控制方法的策略。该策略包括三个核心步骤，即TE的化学图谱分析、ECC的发现及体内过程研究，以及基于ECC的质量标准化。运用此策略，在细胞模型和大鼠心肌缺血再灌注模型中与TE效果相当的四种丹参酮（丹参酮IIA、隐丹参酮、丹参酮I和二氢丹参酮I）的组合被确定为TE中的ECC。此外，基于所提出的ECC，采用多成分测定的单标准（SSDMC）方法建立了TE的质量标准。总之，目前开发的基于ECC的方法不仅为理解草药的整体效应提供了新的视角，也为植物药的质量控制提供了与功效相关的标记成分。
