Qin Jiabang, Huang Zhongbing, Yin Guangfu, Yang Anneng, Han Wei
J Nanosci Nanotechnol. 2016 Mar;16(3):2371-7. doi: 10.1166/jnn.2016.10918.
The conducting composite scaffold, including fiber-cores of aligned poly(L-lactic acid) (PLLA) and shell-layer of polypyrrole (PPy), was fabricated, and then bovine serum albumin (BSA) was conjugated on the PPy shell-layer. Aligned PLLA fibers (about 300 nm diameter) were obtained by electrospinning and rotating drum collection, and then coated by PPy nanoparticles (NPs, about 50 nm diameter) via chemical oxidation. The surface resistivity of PPy-PLLA fibers film were 0.971, 0.874 kΩ. cm at the fiber's vertical and parallel directions, respectively. The results of PPy-PLLA fibers film immersed in phosphate buffer saline for 8 d indicated that the fibers morphology and the film conductivity were not significantly changed, and the fluorescent images showed that FITC-labeled BSA (FITC-BSA) were successfully conjugated in the fibers film with carbodiimide chemistry, and the largest amount of FITC-BSA conjugated in the fibers film from 100 μg/mL proteins solution was 31.31 μg/cm2 due to lots of poly(glutamic acid) in surface-nanogrooves of the fibers surface. Under electrical stimulation of 100 mV, the fibers film was accompanied the release of all conjugated FITC-BSA with the detachment of some PPy NPs. These results suggested that PPy-PLLA fibers film would be potentially applied in the construction of degradable tissue engineering scaffold with protein factors, especially neurotrophic factors for nerve tissue repair.
制备了一种导电复合支架，其包括排列的聚（L-乳酸）（PLLA）纤维芯和聚吡咯（PPy）壳层，然后将牛血清白蛋白（BSA）偶联到PPy壳层上。通过静电纺丝和转鼓收集获得排列的PLLA纤维（直径约300nm），然后通过化学氧化用PPy纳米颗粒（NPs，直径约50nm）进行包覆。PPy-PLLA纤维膜在纤维的垂直和平行方向上的表面电阻率分别为0.971、0.874kΩ·cm。将PPy-PLLA纤维膜浸入磷酸盐缓冲盐水中8天的结果表明，纤维形态和膜电导率没有明显变化，荧光图像显示，通过碳二亚胺化学法，异硫氰酸荧光素标记的BSA（FITC-BSA）成功偶联在纤维膜中，由于纤维表面纳米凹槽中有大量聚谷氨酸，从100μg/mL蛋白质溶液中偶联到纤维膜中的FITC-BSA的最大量为31.31μg/cm²。在100mV的电刺激下，纤维膜伴随着一些PPy NPs的脱离而释放出所有偶联的FITC-BSA。这些结果表明，PPy-PLLA纤维膜在构建具有蛋白质因子，特别是用于神经组织修复的神经营养因子的可降解组织工程支架方面具有潜在应用价值。
