Reversed-phase chromatography of novel biologically effective aminoacridine-N-glycosides by OPLC.

The reversed-phase chromatographic behavior of novel biologically active aminoacridine-N-glycosides is studied. The chromatographic experiments are performed with overpressurized layer chromatography. Weak ion pairs are formed with methanesulfonic acid, but only at low concentrations of the ion-pairing reagent. The retention seems to involve a reversed-phase mechanism. The base compounds only slightly modify the retention, while the number and polarity of the substituents have larger effects. The pH dependence of the retention is very typical for the aminoacridine-N-glycosides, and it plays an important role in the separation. The monoglycosides are completely separated from the corresponding base compounds, as are the diglycosides from the monoglycosides, on RP-2, RP-8, and RP-18 layers with eluents containing 30 to 60% acetonitrile and at least 0.005 M ammonium carbonate at pH 4 to 6.