Plakas S M, el Said K R, Jester E L, Bencsath F A, Hayton W L
U.S. Food and Drug Administration, Gulf Coast Seafood Laboratory, Dauphin Island, AL 36528, USA.
J AOAC Int. 1997 May-Jun;80(3):486-90.
A liquid chromatographic (LC) method was developed for determination of acriflavine (ACR) and proflavine (PRO) residues in channel catfish muscle. Residues were extracted with acidified methanol solution, and extracts were cleaned up with C18 solid-phase extraction columns. Residue concentrations were determined on an LC cyano column, with spectrophotometric detection at 454 nm. Catfish muscle was individually fortified with ACR (purified from commercial product) and PRO at concentrations of 5, 10, 20, 40, and 80 ppb (5 replicates per level). Mean recoveries from fortified muscle at each level ranged from 86 to 95%, with relative standard deviations (RSDs) of 2.5 to 5.7%. The method was applied to incurred residues of ACR and PRO in muscle after waterborne exposure of channel catfish to commercial acriflavine (10 ppm total dye for 4 h). RSDs for incurred residues of ACR and PRO were in the same range as those for fortified muscle. Low residue concentrations (< 1% of exposure water concentration) suggested poor absorption of ACR and PRO in catfish.
建立了一种液相色谱（LC）法，用于测定斑点叉尾鮰肌肉中吖啶黄（ACR）和普罗黄素（PRO）的残留量。残留量用酸化甲醇溶液提取，提取物用C18固相萃取柱净化。在LC氰基柱上测定残留浓度，在454nm处进行分光光度检测。斑点叉尾鮰肌肉分别用浓度为5、10、20、40和80 ppb的ACR（从商业产品中纯化）和PRO进行强化（每个水平5个重复）。每个水平强化肌肉的平均回收率为86%至95%，相对标准偏差（RSD）为2.5%至5.7%。该方法应用于斑点叉尾鮰在水中接触商业吖啶黄（总染料浓度为10 ppm，持续4小时）后肌肉中ACR和PRO的实际残留量测定。ACR和PRO实际残留量的RSD与强化肌肉的RSD范围相同。低残留浓度（<暴露水浓度的1%）表明ACR和PRO在斑点叉尾鮰中的吸收较差。
