State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China.
Veterinary Research Institute, Xinjiang Academy of Animal Science, Urumqi, Xinjiang, 830000, China.
Parasit Vectors. 2016 Jul 25;9(1):411. doi: 10.1186/s13071-016-1687-y.
Alveolar echinococcosis (AE) is a lethal zoonosis caused by the fox-tapeworm Echinococcus multilocularis. The disease is difficult to treat and an effective therapeutic drug is urgently needed. Reliable models are essential for drug development. In this study, we developed both in vitro and in vivo models of larval E. multilocularis.
The protoscoleces (PSC) of E. multilocularis from jirds were successfully cultured in a modified RPMI1640 based medium containing 25 % (v/v) fetal bovine serum (FBS). After 100 days of culture, PSC developed to larval vesicles (small unilocular cysts) and the fast growing vesicles produced PSC in brood capsules. In addition, mice were intraperitoneally injected with 30 cultured small vesicles and 100 % of the mice had resulting metacestode masses.
Larval protoscoleces and vesicles of E. multilocularis grow healthily in vitro in the RPMI1640 based medium containing 25 % FBS. Echinococcus multilocularis in vitro and in vivo models provide a valuable platform for investigating the biology of the parasite and screening effective therapeutic drugs against AE.
泡状棘球蚴病（AE）是一种由狐狸绦虫多房棘球绦虫引起的致命人畜共患病。这种疾病很难治疗，急需有效的治疗药物。可靠的模型对于药物开发至关重要。在这项研究中，我们开发了多房棘球蚴幼虫的体外和体内模型。
从小鼠体内分离出的多房棘球蚴原头蚴（PSC）在含有 25%（v/v）胎牛血清（FBS）的改良 RPMI1640 基础培养基中成功培养。培养 100 天后，PSC 发育成幼虫囊泡（小的单房囊肿），快速生长的囊泡在育囊内产生 PSC。此外，小鼠经腹腔注射 30 个培养的小囊泡，100%的小鼠产生了继生性包虫囊肿。
含有 25% FBS 的 RPMI1640 基础培养基中，多房棘球蚴幼虫原头蚴和囊泡在体外健康生长。多房棘球蚴的体外和体内模型为研究寄生虫生物学和筛选抗 AE 的有效治疗药物提供了有价值的平台。
