在小鼠卵母细胞成熟过程中，Tristetraprolin在细胞骨架组织中发挥作用。
Tristetraprolin functions in cytoskeletal organization during mouse oocyte maturation.
作者信息
Liu Xiaohui, Li Xiaoyan, Ma Rujun, Xiong Bo, Sun Shao-Chen, Liu Honglin, Gu Ling
机构信息
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Center of Reproductive Medicine, Jinling Hospital, Nanjing, China.
出版信息
Oncotarget. 2016 Aug 16;7(33):53330-53338. doi: 10.18632/oncotarget.10755.
Tristetraprolin (TTP), a member of TIS11 family containing CCCH tandem zinc finger, is one of the best characterized RNA-binding proteins. However, to date, the role of TTP in mammalian oocytes remains completely unknown. In the present study, we report the altered maturational progression and cytokinesis, upon specific knockdown of TTP in mouse oocytes. Furthermore, by confocal scanning, we observe the failure to form cortical actin cap during meiosis of TTP-depleted oocytes. Loss of TTP in oocytes also results in disruption of meiotic spindle morphology and chromosome alignment. In support of these findings, incidence of aneuploidy is accordingly increased when TTP is abated in oocytes. Our results suggest that TTP as a novel cytoskeletal regulator is required for spindle morphology/chromosome alignment and actin polymerization in oocytes.
锌指蛋白Tristetraprolin（TTP）是TIS11家族的成员之一，含有CCCH串联锌指结构，是特征最为明确的RNA结合蛋白之一。然而，迄今为止，TTP在哺乳动物卵母细胞中的作用仍完全未知。在本研究中，我们报告了在小鼠卵母细胞中特异性敲低TTP后，其成熟进程和胞质分裂发生了改变。此外，通过共聚焦扫描，我们观察到TTP缺失的卵母细胞在减数分裂过程中未能形成皮质肌动蛋白帽。卵母细胞中TTP的缺失还导致减数分裂纺锤体形态和染色体排列紊乱。支持这些发现的是，当卵母细胞中TTP减少时，非整倍体的发生率相应增加。我们的结果表明，TTP作为一种新型的细胞骨架调节因子，是卵母细胞纺锤体形态/染色体排列和肌动蛋白聚合所必需的。
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