Department of Oral and Maxillofacial Surgery, Daejeon Dental Hospital, Wonkwang University, Daejeon, Korea.
Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.
Clin Oral Implants Res. 2017 Sep;28(9):1164-1172. doi: 10.1111/clr.12933. Epub 2016 Jul 26.
Rapid and stable fixation of dental implants is crucial for successful treatment. Herein, we examined whether the simultaneous treatment of titanium implants with ultraviolet (UV) and alendronate (ALN) synergistically improved the bone-to-implant contact.
We assessed the in vitro effects of UV radiation-treated (UV+/ALN-), ALN-soaked (UV-/ALN+), and UV radiation/ALN-treated (UV+/ALN+) titanium implants on cell proliferation, cytotoxicity, cell adhesion, and osteoblast differentiation using MG-63 osteoblast-like cells by the assays of MTS, live/dead, scanning electron microscopy (SEM), alkaline phosphatase (ALP) activity, and alizarin red S (AR-S) staining, respectively. Furthermore, in vivo bone formation at the bone-implant interface efficiency determined using a rabbit tibia implantation. Implants were divided into 3 experimental groups (UV+/ALN-, UV-/ALN+, UV+/ALN+) and the non-treated control (UV-/ALN-) group and transplanted into the proximal tibia of rabbits. At 1, 2, 4, and 8 weeks post-operation, bone formation at the bone-implant interface was evaluated by micro-computed tomography and histological analysis.
MG-63 cells cultured on UV+/ALN+ implants showed significantly higher cell proliferation, ALP activity, and calcium mineralization than those cultured on other implants (P < 0.05). Furthermore, SEM observation showed the highest increase in cell attachment and growth on the UV+/ALN+ implants. In vivo, experimental groups at all time points showed greater peri-implant bone formation than the control group. At 8 weeks post-implantation, in the UV+/ALN+ group, significantly higher bone formation was observed than the UV+/ALN- or UV-/ALN+ group, respectively (P < 0.05).
Treatment of titanium surfaces with UV and ALN may synergistically enhance osteoblastic differentiation and mineralization in vitro and enhance bone formation at the bone-implant interface in vivo. These data suggest that UV and ALN treatment may improve the osseointegration of titanium implants.
牙种植体的快速和稳定固定对于成功治疗至关重要。在此，我们研究了同时用紫外线（UV）和阿仑膦酸钠（ALN）处理钛种植体是否协同改善了骨-种植体接触。
我们通过 MTS、活/死、扫描电子显微镜（SEM）、碱性磷酸酶（ALP）活性和茜素红 S（AR-S）染色分别评估了经紫外线辐射处理（UV+/ALN-）、ALN 浸泡（UV-/ALN+）和紫外线/ALN 处理（UV+/ALN+）的钛种植体对 MG-63 成骨样细胞增殖、细胞毒性、细胞黏附及成骨分化的体外影响。此外，还通过兔胫骨植入物评估了骨-种植体界面的体内骨形成效率。将植入物分为 3 个实验组（UV+/ALN-、UV-/ALN+、UV+/ALN+）和未处理的对照组（UV-/ALN-），并移植到兔的胫骨近端。术后 1、2、4 和 8 周，通过微计算机断层扫描和组织学分析评估骨-种植体界面的骨形成。
在 UV+/ALN+种植体上培养的 MG-63 细胞的细胞增殖、ALP 活性和钙矿化明显高于其他种植体（P<0.05）。此外，SEM 观察显示细胞黏附和生长的增加最明显。在体内，各时间点实验组的种植体周围骨形成均高于对照组。植入后 8 周，与 UV+/ALN-或 UV-/ALN+组相比，UV+/ALN+组的骨形成明显更高（P<0.05）。
用紫外线和 ALN 处理钛表面可能在体外协同增强成骨细胞分化和矿化，并在体内增强骨-种植体界面的骨形成。这些数据表明，紫外线和 ALN 处理可能会改善钛种植体的骨整合。
