Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Sensengasse 2a, A-1090, Vienna, Austria.
Division of Oral Surgery, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.
BMC Oral Health. 2020 Apr 25;20(1):125. doi: 10.1186/s12903-020-01089-4.
Bisphosphonate coating of dental implants is a promising tool for surface modification aiming to improve the osseointegration process and clinical outcome. The biological effects of bisphosphonates are thought to be mainly associated with osteoclasts inhibition, whereas their effects on osteoblast function are unclear. A potential of bisphosphonate coated surfaces to stimulate osteoblast differentiation was investigated by several in vitro studies with contradictory results. The purpose of this systematic review and meta-analysis was to evaluate the effect of bisphosphonate coated implant surfaces on alkaline phosphatase activity in osteoblasts.
In vitro studies that assessed alkaline phosphatase activity in osteoblasts following cell culture on bisphosphonate coated titanium surfaces were searched in electronic databases PubMed/MEDLINE, Scopus and ISI Web of Science. Animal studies and clinical trials were excluded. The literature search was restricted to articles written in English and published up to August 2019. Publication bias was assessed by the construction of funnel plots.
Eleven studies met the inclusion criteria. Meta-analysis showed that coating of titanium surfaces with bisphosphonates increases alkaline phosphatase activity in osteoblasts after 3 days (n = 1), 7 (n = 7), 14 (n = 6) and 21 (n = 3) days. (7 days beta coefficient = 1.363, p-value = 0.001; 14 days beta coefficient = 1.325, p-value < 0.001; 21 days beta coefficient = 1.152, p-value = 0.159).
The meta-analysis suggests that bisphosphonate coatings of titanium implant surfaces may have beneficial effects on osteogenic behaviour of osteoblasts grown on titanium surfaces in vitro. Further studies are required to assess to which extent bisphosphonates coating might improve osseointegration in clinical situations.
在口腔种植领域，通过在种植体表面涂覆双膦酸盐来改善种植体的表面特性，从而提高种植体的骨整合效果，这是一种很有前途的表面修饰方法。双膦酸盐的生物学效应主要与破骨细胞抑制有关，但其对成骨细胞功能的影响尚不清楚。已有体外研究表明，双膦酸盐涂层表面具有刺激成骨细胞分化的潜力，但研究结果存在差异。本系统评价和荟萃分析旨在评估双膦酸盐涂层种植体表面对成骨细胞碱性磷酸酶活性的影响。
我们在电子数据库 PubMed/MEDLINE、Scopus 和 ISI Web of Science 中检索了评估双膦酸盐涂层钛表面培养的成骨细胞碱性磷酸酶活性的体外研究。排除了动物研究和临床试验。文献检索仅限于用英文撰写并于 2019 年 8 月前发表的文章。通过构建漏斗图评估发表偏倚。
11 项研究符合纳入标准。荟萃分析显示，双膦酸盐涂层钛表面可在第 3 天（n=1）、第 7 天（n=7）、第 14 天（n=6）和第 21 天（n=3）时增加成骨细胞的碱性磷酸酶活性。（第 7 天的β系数=1.363，p 值=0.001；第 14 天的β系数=1.325，p 值<0.001；第 21 天的β系数=1.152，p 值=0.159）。
荟萃分析表明，双膦酸盐涂层可能对体外培养的成骨细胞在钛表面的成骨行为具有有益影响。需要进一步研究来评估双膦酸盐涂层在临床情况下对骨整合的改善程度。
