Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory , 7000 East Avenue, Livermore, California 94550, United States.
Biology and Biotechnology Division, Lawrence Livermore National Laboratory , 7000 East Avenue, Livermore, California 94550, United States.
Anal Chem. 2016 Sep 6;88(17):8714-9. doi: 10.1021/acs.analchem.6b02054. Epub 2016 Aug 9.
A cavity ring-down spectroscopy (CRDS) instrument was developed using mature, robust hardware for the measurement of carbon-14 in biological studies. The system was characterized using carbon-14 elevated glucose samples and returned a linear response up to 387 times contemporary carbon-14 concentrations. Carbon-14 free and contemporary carbon-14 samples with varying carbon-13 concentrations were used to assess the method detection limit of approximately one-third contemporary carbon-14 levels. Sources of inaccuracies are presented and discussed, and the capability to measure carbon-14 in biological samples is demonstrated by comparing pharmacokinetics from carbon-14 dosed guinea pigs analyzed by both CRDS and accelerator mass spectrometry. The CRDS approach presented affords easy access to powerful carbon-14 tracer techniques that can characterize complex biochemical systems.
开发了一种基于成熟、可靠硬件的腔衰荡光谱（CRDS）仪器，用于生物研究中碳-14 的测量。该系统使用碳-14 升高的葡萄糖样品进行了表征，其线性响应高达当代碳-14 浓度的 387 倍。使用无碳-14 和当代碳-14 样品以及不同碳-13 浓度的样品来评估方法检测限约为当代碳-14 水平的三分之一。本文介绍并讨论了误差的来源，并通过比较经 CRDS 和加速器质谱分析的碳-14 标记豚鼠的药代动力学，证明了该方法在测量生物样品中碳-14 的能力。所提出的 CRDS 方法为使用强大的碳-14 示踪技术来描述复杂的生化系统提供了便捷途径。
