Nguyen Truong Xuan, Lee Sung-Il, Rai Rameshwar, Kim Nam-Soo, Kim Jong Hwa
a Oriental Bio-herb Research Institute, Kangwon National University, Chuncheon 200-701, Korea.
b Institute of Agro-Biology, Vietnam National University of Agriculture, Hanoi, Vietnam.
Genome. 2016 Aug;59(8):551-64. doi: 10.1139/gen-2016-0011. Epub 2016 Jun 17.
Lilium lancifolium Thunb. (2n = 2x = 24) is a cytologically conspicuous species with both diploids and triploids in nature. Cytological and molecular genetic analyses were carried out in both diploids and triploids that were collected from 55 geographical locations in Korea, Japan, and China. While the 5S rRNA gene loci were located at duplicated loci on the long arm of chromosome 2, the 45S rRNA gene loci were present in chromosomes 1, 2, 4, 6, 7, and 11. While the loci on chromosomes 1 and 7 were constant, the loci on chromosomes 2, 4, 6, 7, and 11 were variable in some plants so that the L. lancifolium accessions were grouped into 7 cytotypes in diploids and 12 cytotypes in triploids. REMAP marker analysis revealed that the diploids were classified into seven clusters, and the triploids were classified into a large cluster. Geographic, cytological, and genetic differentiations were not related in both the diploid and triploid accessions of L. lancifolium. Thus, current genetic variations occurred prior to the geographic differentiation in both diploids and triploids, and the 45S rDNA cytotype variations occurred after geographic differentiation in the current habitats of L. lancifolium.
卷丹（Lilium lancifolium Thunb.，2n = 2x = 24）是一种在细胞学上较为显著的物种，在自然状态下既有二倍体也有三倍体。对从韩国、日本和中国55个地理位置采集的二倍体和三倍体进行了细胞学和分子遗传学分析。5S rRNA基因位点位于第2号染色体长臂的重复位点上，而45S rRNA基因位点存在于第1、2、4、6、7和11号染色体上。虽然第1和7号染色体上的位点是恒定的，但在一些植株中，第2、4、6、7和11号染色体上的位点是可变的，因此卷丹材料在二倍体中被分为7种细胞型，在三倍体中被分为12种细胞型。REMAP标记分析表明，二倍体被分为7个簇，三倍体被分为一个大簇。卷丹的二倍体和三倍体材料在地理、细胞学和遗传分化方面均无关联。因此，当前的遗传变异在二倍体和三倍体的地理分化之前就已发生，而45S rDNA细胞型变异在卷丹当前栖息地的地理分化之后发生。
