Montero Juan F D, Barbosa Luiz C A, Pereira Ulisses A, Barra Guilherme M, Fredel Márcio C, Benfatti Cesar A M, Magini Ricardo S, Pimenta Andréa L, Souza Júlio C M
Center for Research on Dental Implants (CEPID), School of Dentistry (ODT), Federal University of Santa Catarina (UFSC), Florianópolis/SC, 88040-900, Brazil.
Department of Chemistry, Federal University of Minas Gerais (UFMG), Av. Pres. Antônio Carlos, 6627, Campus Pampulha, CEP 31270-901, Belo Horizonte, MG, Brazil.
J Biomed Mater Res A. 2016 Dec;104(12):3015-3020. doi: 10.1002/jbm.a.35842. Epub 2016 Aug 5.
Poly-ether-ether-ketone (PEEK) is currently introduced as an alternative material for orthopedic implants due to its biocompatibility and low elastic modulus compared to titanium. Also, a sulphonation treatment can functionalize PEEK to embed therapeutical substances. The objective of this work was to functionalize a PEEK film to incorporate novel lactam-based antibiofilms compounds. PEEK samples were functionalized by sulphuric acid treatment and then dissolved in dimethylsulfoxide, where lactams were added to be incorporated into the polymer. A dip-coating technique was used to synthesize a thin film on a glass-based substrate. The degree of sulfonation (DS) and the incorporation of lactams into sulphonated PEEK (sPEEK) were analyzed by Fourier transform infrared spectroscopy, nuclear magnetic resonance, thermogravimetric analysis (TGA), and scanning electron microscopy. A DS of 65% was obtained and TGA curves confirmed the presence of SO H and lactams in the sPEEK structure. The growth of Streptococcus mutans biofilm decreased on sPEEK surface containing lactams when compared to sPEEK free of lactams. That indicated the antibiofilm activity of those compounds was maintained after incorporation into sPEEK. Planktonic growth analysis showed no long distant effects of sPEEK containing lactams, indicating that no systemic effects should be expected upon clinical uses of medical devices produced with lactam-treated sPEEK. Results revealed that inclusion of lactams into sPEEK represents a good alternative for the production of biomaterials resistant to bacterial accumulation. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 3015-3020, 2016.
聚醚醚酮（PEEK）由于其生物相容性以及与钛相比更低的弹性模量，目前被引入作为骨科植入物的替代材料。此外，磺化处理可以使PEEK功能化以嵌入治疗物质。这项工作的目的是使PEEK薄膜功能化，以纳入新型内酰胺类抗生物膜化合物。通过硫酸处理使PEEK样品功能化，然后将其溶解在二甲基亚砜中，向内酰胺中加入内酰胺以使其掺入聚合物中。采用浸涂技术在玻璃基衬底上合成薄膜。通过傅里叶变换红外光谱、核磁共振、热重分析（TGA）和扫描电子显微镜分析磺化度（DS）以及内酰胺掺入磺化PEEK（sPEEK）的情况。获得了65%的DS，TGA曲线证实了sPEEK结构中存在-SO₃H和内酰胺。与不含内酰胺的sPEEK相比，含内酰胺的sPEEK表面变形链球菌生物膜的生长减少。这表明这些化合物在掺入sPEEK后仍保持抗生物膜活性。浮游生长分析表明含内酰胺的sPEEK没有远距离影响，这表明在用内酰胺处理的sPEEK生产的医疗器械临床使用时不应预期有全身影响。结果表明，将内酰胺掺入sPEEK是生产抗细菌积聚生物材料的一个很好的选择。© 2016威利期刊公司。《生物医学材料研究杂志》A部分：104A：3015 - 3020，2016年。
