Marti Elisabet, Huerta Belinda, Rodríguez-Mozaz Sara, Barceló Damià, Balcázar Jose Luis, Marcé Rafael
Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, Emili Grahit 101, 17003 Girona, Spain.
Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, Emili Grahit 101, 17003 Girona, Spain; Department of Environmental Chemistry, IDAEA-CSIC, C/ Jordi Girona 18-26, 08034 Barcelona, Spain.
Chemosphere. 2016 Oct;161:470-474. doi: 10.1016/j.chemosphere.2016.07.054. Epub 2016 Jul 25.
We used a short-term microcosm approach to investigate the influence of two different subinhibitory concentrations of ciprofloxacin (0.01 and 0.1 μg/ml) on both the abundance of a plasmid-mediated quinolone resistance determinant (qnrS) and the structure and composition of bacterial communities from impaired and pristine water supply reservoirs. The results showed that the abundance of the qnrS gene increases in water samples exposed to both subinhibitory concentrations of ciprofloxacin, especially in water samples from La Llosa del Cavall, which represents the pristine system. Subinhibitory ciprofloxacin concentrations also induced changes in bacterial community composition as indicated by the relative abundances of each operational taxonomic unit (OTU) across treatments. Therefore, our findings may be of significant importance because subinhibitory ciprofloxacin concentrations may promote antibiotic resistance and affect bacterial community composition in environmental settings.
我们采用短期微观世界方法，研究了两种不同亚抑制浓度的环丙沙星（0.01和0.1μg/ml）对质粒介导的喹诺酮抗性决定子（qnrS）丰度以及受损和原始供水水库中细菌群落结构与组成的影响。结果表明，暴露于两种亚抑制浓度环丙沙星的水样中，qnrS基因的丰度增加，尤其是在代表原始系统的拉洛萨德尔卡瓦尔的水样中。各处理间每个可操作分类单元（OTU）的相对丰度表明，亚抑制浓度的环丙沙星也会诱导细菌群落组成发生变化。因此，我们的研究结果可能具有重要意义，因为亚抑制浓度的环丙沙星可能会促进抗生素抗性并影响环境中的细菌群落组成。
