Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003, Girona, Spain; BETA Technological Center, Faculty of Science and Technology, University of Vic-Central University of Catalonia, 08500, Vic, Spain.
Catalan Institute for Water Research (ICRA), Emili Grahit 101, 17003, Girona, Spain.
Chemosphere. 2018 Apr;196:115-119. doi: 10.1016/j.chemosphere.2017.12.108. Epub 2017 Dec 18.
This study was aimed to determine the abundance of four antibiotic resistance genes (bla, ermB, qnrS and sulI), as well as bacterial community composition associated with the intestinal mucus of wild freshwater fish species collected from the Foix and La Llosa del Cavall reservoirs, which represent ecosystems with high and low anthropogenic disturbance, respectively. Water and sediments from these reservoirs were also collected and analyzed to determine the pollution level by antibiotics. The bla gene was only detected in brown trout and Ebro barbel, which were collected from La Llosa del Cavall reservoir. In contrast, the sulI and qnrS genes were only detected in common carp, which were collected from the Foix reservoir. Although the ermB gene was also detected in common carp, the values were below the limit of quantification. Likewise, water and sediment samples from the Foix reservoir had higher concentrations and more classes of antibiotics than those from La Llosa del Cavall. Pyrosequencing analysis of 16S rRNA genes revealed significant differences in bacterial communities associated with the intestinal mucus of fish species. Therefore, these findings suggest that anthropogenic activities are not only increasing the pollution of aquatic environments, but also contributing to the emergence and spread of antibiotic resistance in organisms that inhabit such environments.
本研究旨在确定来自 Foix 和 La Llosa del Cavall 水库的野生淡水鱼类肠道粘液中与抗生素耐药基因（bla、ermB、qnrS 和 sulI）相关的细菌群落组成。这两个水库分别代表了人为干扰程度高和低的生态系统。还收集和分析了这些水库的水和沉积物，以确定抗生素的污染水平。bla 基因仅在 La Llosa del Cavall 水库采集的褐鳟和欧鲶中检测到。相比之下，sulI 和 qnrS 基因仅在 Foix 水库采集的鲤鱼中检测到。虽然 ermB 基因也在鲤鱼中检测到，但含量低于定量限。同样，来自 Foix 水库的水和沉积物样本的抗生素浓度和种类均高于来自 La Llosa del Cavall 的水库。16S rRNA 基因的焦磷酸测序分析显示，鱼类肠道粘液相关细菌群落存在显著差异。因此，这些发现表明，人为活动不仅增加了水生环境的污染，还导致了栖息在这些环境中的生物体中抗生素耐药性的出现和传播。
