Szewczyk A, Nałecz M J, Wojtczak L
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland.
J Biochem Biophys Methods. 1989 Mar;18(2):125-34. doi: 10.1016/0165-022x(89)90074-2.
New photoaffinity probes, N-(4-azidosalicylic)-aminosuccinic acid, 3-(4-azidophenylazo)-4-hydroxyphenylmalonic acid, (4-azido-2-nitroanilino)-N-succinic acid, 4-azidophenacylthiosuccinic acid and 4-azidophenylsuccinic acid, were synthesized and characterized chemically. They differ in the distance between dicarboxylic and azido groups, hydrophobicity and acidic moiety. These between dicarboxylic and azido groups, hydrophobicity and acidic moiety. These reagents can be applied for photoaffinity labeling of mitochondrial anion carriers and enzymes interacting with dicarboxylic acids. Inhibition and labeling of the dicarboxylate carrier is presented.
合成了新型光亲和探针N-(4-叠氮基水杨酸)-氨基琥珀酸、3-(4-叠氮基苯偶氮)-4-羟基苯基丙二酸、(4-叠氮基-2-硝基苯胺)-N-琥珀酸、4-叠氮苯甲酰硫代琥珀酸和4-叠氮基苯基琥珀酸，并进行了化学表征。它们在二羧酸和叠氮基之间的距离、疏水性和酸性部分存在差异。这些试剂可用于线粒体阴离子载体和与二羧酸相互作用的酶的光亲和标记。本文展示了二羧酸载体的抑制和标记情况。
