Basiji David A
Department of Bioengineering, University of Washington, Seattle, WA, USA.
Methods Mol Biol. 2016;1389:13-21. doi: 10.1007/978-1-4939-3302-0_2.
Over the last decade imaging flow cytometry (IFC) has become an established technique, as evidenced by its use in over 500 peer-reviewed scientific articles. Nevertheless, it is still an emerging technique with an installed base of less than 5 % that of conventional flow cytometers. In parallel with its adoption, the technology has evolved rapidly, increasing in speed, sensitivity, and real-time data analysis capacity by over an order of magnitude since its introduction. This chapter summarizes IFC's basic principles of operation and describes the current state of the art.
在过去十年中，成像流式细胞术（IFC）已成为一项成熟的技术，500多篇同行评审的科学文章对其的使用就证明了这一点。然而，它仍然是一项新兴技术，装机量不到传统流式细胞仪的5%。随着该技术的应用，它也在迅速发展，自推出以来，其速度、灵敏度和实时数据分析能力提高了一个多数量级。本章总结了IFC的基本操作原理，并描述了其当前的技术水平。
