Amnis成像流式细胞术原理

Principles of Amnis Imaging Flow Cytometry.

作者信息

Basiji David A

机构信息

Department of Bioengineering, University of Washington, Seattle, WA, USA.

出版信息

Methods Mol Biol. 2016;1389:13-21. doi: 10.1007/978-1-4939-3302-0_2.

DOI:10.1007/978-1-4939-3302-0_2

PMID:27460235

Abstract

Over the last decade imaging flow cytometry (IFC) has become an established technique, as evidenced by its use in over 500 peer-reviewed scientific articles. Nevertheless, it is still an emerging technique with an installed base of less than 5 % that of conventional flow cytometers. In parallel with its adoption, the technology has evolved rapidly, increasing in speed, sensitivity, and real-time data analysis capacity by over an order of magnitude since its introduction. This chapter summarizes IFC's basic principles of operation and describes the current state of the art.

摘要

在过去十年中，成像流式细胞术（IFC）已成为一项成熟的技术，500多篇同行评审的科学文章对其的使用就证明了这一点。然而，它仍然是一项新兴技术，装机量不到传统流式细胞仪的5%。随着该技术的应用，它也在迅速发展，自推出以来，其速度、灵敏度和实时数据分析能力提高了一个多数量级。本章总结了IFC的基本操作原理，并描述了其当前的技术水平。