成像流式细胞术中的掩膜
Masks in imaging flow cytometry.
作者信息
Dominical Venina, Samsel Leigh, McCoy J Philip
机构信息
National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, United States.
National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, United States.
出版信息
Methods. 2017 Jan 1;112:9-17. doi: 10.1016/j.ymeth.2016.07.013. Epub 2016 Jul 25.
Abstract
Data analysis in imaging flow cytometry incorporates elements of flow cytometry together with other aspects of morphological analysis of images. A crucial early step in this analysis is the creation of a mask to distinguish the portion of the image upon which further examination of specified features can be performed. Default masks are provided by the manufacturer of the imaging flow cytometer but additional custom masks can be created by the individual user for specific applications. Flawed or inaccurate masks can have a substantial negative impact on the overall analysis of a sample, thus great care must be taken to ensure the accuracy of masks. Here we discuss various types of masks and cite examples of their use. Furthermore we provide our insight for how to approach selecting and assessing the optimal mask for a specific analysis.
摘要
成像流式细胞术的数据分析将流式细胞术的要素与图像形态学分析的其他方面结合在一起。该分析中一个关键的早期步骤是创建一个掩膜，以区分图像中可对指定特征进行进一步检查的部分。成像流式细胞仪的制造商提供默认掩膜，但个人用户可针对特定应用创建额外的自定义掩膜。有缺陷或不准确的掩膜可能会对样本的整体分析产生重大负面影响，因此必须格外小心以确保掩膜的准确性。在此，我们讨论各种类型的掩膜并列举其使用示例。此外，我们还提供关于如何为特定分析选择和评估最佳掩膜的见解。
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