Pierzchalski Arkadiusz, Mittag Anja, Tárnok Attila
Department of Pediatric Cardiology, Heart Centre, University of Leipzig, Germany.
Methods Cell Biol. 2011;102:1-21. doi: 10.1016/B978-0-12-374912-3.00001-8.
Cytometric techniques are continually being improved, refined, and adapted to new applications. This chapter briefly outlines recent advances in the field of cytometry with the main focus on new instrumentations in flow and image cytometry as well as new probes suitable for multiparametric analyses. There is a remarkable trend for miniaturizing cytometers, developing label-free and fluorescence-free analytical approaches, and designing "intelligent" probes. Furthermore, new methods for analyzing complex data for extracting relevant information are reviewed.
细胞计数技术不断得到改进、完善，并适用于新的应用。本章简要概述了细胞计数领域的最新进展，主要关注流式细胞术和图像细胞术的新仪器以及适用于多参数分析的新探针。细胞计数器小型化、开发无标记和无荧光分析方法以及设计“智能”探针的趋势显著。此外，还综述了分析复杂数据以提取相关信息的新方法。
