Skubitz K M, Christiansen N P, Mendiola J R
Department of Medicine, University of Minnesota Medical School, Minneapolis.
J Leukoc Biol. 1989 Aug;46(2):109-18. doi: 10.1002/jlb.46.2.109.
This report describes the production and characterization of five murine monoclonal antibodies that react with granule proteins of human granulocytes. Monoclonal antibody AHN-11 (IgG2a) reacted specifically with neutrophil cathepsin G; no reactivity with the homologous neutrophil neutral proteases, elastase, proteinase 3, or esterase N was detected. Antibodies AHN-9 (IgG1) and AHN-9.1 (IgG2b) each reacted with different epitopes on human lactoferrin, but not with the homologous protein transferrin. Two IgG1 antibodies, AHE-1 and AHE-2, reacted specifically with eosinophils; AHE-1 reacted strongly with eosinophil peroxidase but not eosinophil major basic protein while AHE-2 recognized eosinophil major basic protein but not eosinophil peroxidase. All five antibodies could detect their respective antigens in alcohol-fixed cytospin preparations. These antibodies should be useful for immunolocalization and quantification of their respective antigens as well as for other studies of the roles of these proteins in granulocyte function and differentiation.
本报告描述了五种与人类粒细胞颗粒蛋白发生反应的鼠单克隆抗体的制备和特性。单克隆抗体AHN-11（IgG2a）与中性粒细胞组织蛋白酶G特异性反应；未检测到与同源中性粒细胞中性蛋白酶、弹性蛋白酶、蛋白酶3或酯酶N的反应性。抗体AHN-9（IgG1）和AHN-9.1（IgG2b）分别与人乳铁蛋白上的不同表位发生反应，但不与同源蛋白转铁蛋白发生反应。两种IgG1抗体AHE-1和AHE-2与嗜酸性粒细胞特异性反应；AHE-1与嗜酸性粒细胞过氧化物酶强烈反应，但不与嗜酸性粒细胞主要碱性蛋白反应，而AHE-2识别嗜酸性粒细胞主要碱性蛋白，但不识别嗜酸性粒细胞过氧化物酶。所有五种抗体均可在酒精固定的细胞涂片制剂中检测到其各自的抗原。这些抗体可用于其各自抗原的免疫定位和定量，以及用于这些蛋白在粒细胞功能和分化中作用的其他研究。
