Sánchez José H, Martínez Humberto A, García María M, Garrido Germán, Gómez Luis, Aguilar José A, de Andrés Damián F, Reina Ramsés, Ramírez Hugo
Laboratorio de Virología, Genética y Biología Molecular, Facultad de Estudios Superiores Cuautitlán (FESC) Campo 4, Universidad Nacional Autónoma de México (UNAM), Estado de México, México.
Laboratorio de Inmuno-virología, Unidad de Investigación Médica en Inmunología, UMAE Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México, México.
Theriogenology. 2016 Nov;86(8):1953-1957.e1. doi: 10.1016/j.theriogenology.2016.06.017. Epub 2016 Jun 23.
The transmission frequency of small ruminant lentiviruses (SRLVs) through the placenta is controversial and may be associated with breed susceptibility. In Mexico, SRLV infections in sheep have been poorly studied. This work explores the presence of antibodies and proviral DNA in Mexican Pelibuey sheep. Enzyme-linked immunosorbent assays (ELISAs; three commercial kits and two on the basis of synthetic peptides) and polymerase chain reaction (PCR; amplifying the long terminal repeat and gag segments) were performed to diagnose SRLV infection in 25 adult Pelibuey ewes with an average age of 2.5 years and 32 fetuses with gestational ages ranging from 40 to 90 days without clinical signs of SRLV. Two of the three commercial ELISAs and the synthetic peptide-based ones were positive for SRLV antibody detection in 28% and 24% of the ewes, respectively, whereas none of the fetuses were positive by any of the ELISAs. By PCR, 31% of the ewes and, interestingly, two fetuses were positive. Characteristic SRLV lesions were not found in the fetal and/or ewe tissues, including those with positive PCR results. These findings demonstrate the susceptibility of Pelibuey sheep to SRLV infection and the low transmission frequency through the placenta.
小反刍兽慢病毒（SRLV）经胎盘传播的频率存在争议，且可能与品种易感性有关。在墨西哥，绵羊的SRLV感染情况研究较少。本研究探讨了墨西哥佩利布埃羊体内抗体和前病毒DNA的存在情况。采用酶联免疫吸附测定（ELISA；三种商用试剂盒和两种基于合成肽的方法）和聚合酶链反应（PCR；扩增长末端重复序列和gag片段）对25只平均年龄为2.5岁的成年佩利布埃母羊和32只胎龄为40至90天且无SRLV临床症状的胎儿进行SRLV感染诊断。三种商用ELISA中的两种以及基于合成肽的方法分别在28%和24%的母羊中检测到SRLV抗体呈阳性，而所有胎儿的ELISA检测均为阴性。通过PCR检测，31%的母羊呈阳性，有趣的是，有两只胎儿也呈阳性。在胎儿和/或母羊组织中未发现典型的SRLV病变，包括PCR结果呈阳性的组织。这些发现表明佩利布埃羊对SRLV感染易感，且经胎盘传播的频率较低。
