Fernando Guerra, Paul Fisch, Laura Jufe, Alejandra Avagnina Marí, Gabriela Mendeluk, Alberto Palaoro Luis
Cytology, Department of Clinical Biochemistry, Clinical Hospital (UBA), INFIBIOC-Argentina, Argentina.
Department of Pathology, Freiburg University Medical Center, Freiburg, Germany.
J Cancer Res Ther. 2016 Apr-Jun;12(2):1075-9. doi: 10.4103/0973-1482.147392.
The loss of reduction of some adhesion molecules is associated with the invasive phenotype of carcinomas. The aim of this paper was to study the expression of some proteins related to the Wnt/β catenin signalling pathway in seminomas by immunohistochemical techniques in order to assess the contribution of this pathway to the tumoral development.
Immunohistochemistry for E-cadherin, β-catenin, vimentin, C-Myc, cyclin D1 (CD1) and placental alkaline phosphatase. (PALP) was carried out in 24 archival tissue blocks of seminomas. Two cellular lines were used as E-cadherin and β-catenin controls. (JKT-1 and TCam-2).
E-cadherin was positive in two seminomas and in one carcinoma in situ. (CIS), showing a membranous pattern. βcatenin was principally expressed in Sertoli cells, in some malignant gonocytes of CIS and in the membranes of seminomatous cells. No β-catenin immunostaining was detectable in the cytoplasm and the nuclei of neoplastic cells. Seminomas were weakly possitive for vimentin in 11/24 cases. None of the tumors displayed expression of C-Myc or CD1.
The results does not indicate the activation of the Wnt pathway, due to the lack of vimentin expression in 13/24 seminomas, the low expression in the rest of the cases, the lack of β-catenin in nuclei and the absence of CD1 and C-Myc expression. Further work is needed to confirm these observations and to test other signaling pathways in seminomas.
某些黏附分子表达的缺失与癌的侵袭表型相关。本文旨在通过免疫组织化学技术研究精原细胞瘤中一些与Wnt/β-连环蛋白信号通路相关蛋白的表达，以评估该信号通路在肿瘤发生发展中的作用。
对24例精原细胞瘤存档组织块进行E-钙黏蛋白、β-连环蛋白、波形蛋白、C-Myc、细胞周期蛋白D1（CD1）和胎盘碱性磷酸酶（PALP）的免疫组织化学检测。使用两种细胞系作为E-钙黏蛋白和β-连环蛋白的对照（JKT-1和TCam-2）。
E-钙黏蛋白在2例精原细胞瘤和1例原位癌（CIS）中呈阳性，呈膜性模式。β-连环蛋白主要表达于支持细胞、CIS的一些恶性生殖母细胞以及精原细胞瘤细胞的细胞膜。在肿瘤细胞的细胞质和细胞核中未检测到β-连环蛋白免疫染色。11/24例精原细胞瘤波形蛋白呈弱阳性。所有肿瘤均未显示C-Myc或CD1的表达。
由于13/24例精原细胞瘤缺乏波形蛋白表达、其余病例表达较低、细胞核中缺乏β-连环蛋白以及未检测到CD1和C-Myc表达，这些结果未表明Wnt信号通路被激活。需要进一步的研究来证实这些观察结果并检测精原细胞瘤中的其他信号通路。
