Suster S, Moran C A, Dominguez-Malagon H, Quevedo-Blanco P
Arkadi M. Rywlin Department of Pathology & Laboratory Medicine, Mount Sinai Medical Center, and University of Miami School of Medicine, FL, USA.
Hum Pathol. 1998 Jul;29(7):737-42. doi: 10.1016/s0046-8177(98)90284-2.
An immunohistochemical study was performed in 120 cases of mediastinal and testicular germ cell tumors from archival, paraffin-embedded material to compare the patterns of expression between the two groups with a panel of markers, including broad-spectrum keratin, CAM 5.2 low-molecular-weight cytokeratin, placental-like alkaline phosphatase (PLAP), alpha-fetoprotein (AFP), human beta-chorionic gonadotropin (hCG), vimentin, and CD30 (Ki-1 antigen). Significant differences were observed between mediastinal and testicular seminomas: mediastinal seminomas showed strong dot-like paranuclear positivity of the tumor cells with antibodies to CAM 5.2 low-molecular-weight keratin in 80% of cases (32 of 40), as compared with only 20% positivity (5 of 24) in testicular seminomas; placental alkaline phosphatase (PLAP) was also found to be less commonly expressed in testicular seminomas (12 of 24) than in mediastinal seminomas (37 of 40); a similar pattern of expression was also observed for vimentin, which was present in scattered tumor cells in a higher proportion of mediastinal seminomas (28 of 40) than in testicular seminomas (11 of 24). The staining pattern and distribution of these markers did not show significant differences between the two groups for the various other tumor categories studied, including yolk sac tumor, embryonal carcinoma, and choriocarcinoma. The tumor cells in both testicular and mediastinal embryonal carcinoma showed strong positivity for the CD30 antigen; however, strong positivity for this marker was also observed in 6 of 25 yolk sac tumors and in scattered individual tumor cells in 4 of 63 seminomas. The results of this study show that significant differences exist between the immunostaining patterns of mediastinal and testicular seminomas, suggesting that the former may be characterized by a more mature phenotype than their testicular counterparts. Also, CD30 expression may not be necessarily restricted to embryonal carcinomas and occasionally may be observed in yolk sac tumors and seminoma cells, supporting the close histogenetic relationship that exists among these tumor types.
对120例存档的石蜡包埋纵隔和睾丸生殖细胞肿瘤进行免疫组化研究，以一组标志物比较两组的表达模式，这些标志物包括广谱角蛋白、CAM 5.2低分子量细胞角蛋白、胎盘样碱性磷酸酶（PLAP）、甲胎蛋白（AFP）、人β绒毛膜促性腺激素（hCG）、波形蛋白和CD30（Ki-1抗原）。在纵隔和睾丸精原细胞瘤之间观察到显著差异：纵隔精原细胞瘤中，80%的病例（40例中的32例）肿瘤细胞对CAM 5.2低分子量角蛋白抗体呈强点状核旁阳性，而睾丸精原细胞瘤中只有20%呈阳性（24例中的5例）；还发现胎盘碱性磷酸酶（PLAP）在睾丸精原细胞瘤（24例中的12例）中的表达不如纵隔精原细胞瘤（40例中的37例）常见；波形蛋白也观察到类似的表达模式，纵隔精原细胞瘤（40例中的28例）中散在肿瘤细胞中的波形蛋白比例高于睾丸精原细胞瘤（24例中的11例）。对于研究的其他各种肿瘤类型，包括卵黄囊瘤、胚胎癌和绒毛膜癌，两组之间这些标志物的染色模式和分布没有显示出显著差异。睾丸和纵隔胚胎癌中的肿瘤细胞对CD30抗原均呈强阳性；然而，在25例卵黄囊瘤中的6例以及63例精原细胞瘤中的4例散在单个肿瘤细胞中也观察到该标志物呈强阳性。本研究结果表明，纵隔和睾丸精原细胞瘤的免疫染色模式存在显著差异，提示前者可能比其睾丸对应物具有更成熟的表型。此外，CD30表达不一定局限于胚胎癌，偶尔也可在卵黄囊瘤和精原细胞瘤细胞中观察到，这支持了这些肿瘤类型之间存在密切的组织发生学关系。
