人肌肉中肌球蛋白重链表达的免疫细胞化学证明

Immunocytochemical demonstration of myosin heavy chain expression in human muscle.

作者信息

Ecob-Prince M, Hill M, Brown W

机构信息

Glasgow University Department of Neurology, Southern General Hospital, U.K.

出版信息

J Neurol Sci. 1989 Jun;91(1-2):71-8. doi: 10.1016/0022-510x(89)90076-2.

DOI:10.1016/0022-510x(89)90076-2

PMID:2746293

Abstract

Three new monoclonal antibodies are shown by immunocytochemical techniques to recognise the adult fast, slow and neonatal myosin heavy chain (MHC) isoforms in adult and fetal human muscle. In fetal muscle of 17-20 weeks of gestation, slow MHC was present only in primary myotubes. Secondary myotubes contained neonatal MHC with different levels of fast and some embryonic MHC. We confirmed the presence of tertiary myotubes in the fetal muscle (Draeger et al. (1987) J. Neurol. Sci., 81: 19-43) and show that these contained fast, neonatal and possibly some embryonic MHC. Fast MHC was therefore present in secondary and tertiary myotubes at least as early as 17 days of gestation.

摘要

免疫细胞化学技术显示，三种新的单克隆抗体可识别成人及胎儿人类肌肉中的成人快肌、慢肌和新生儿肌球蛋白重链（MHC）亚型。在妊娠17 - 20周的胎儿肌肉中，慢肌MHC仅存在于初级肌管中。次级肌管含有不同水平的快肌MHC以及一些胚胎型MHC的新生儿MHC。我们证实了胎儿肌肉中存在三级肌管（Draeger等人，（1987年）《神经科学杂志》，81: 19 - 43），并表明这些三级肌管含有快肌、新生儿型MHC以及可能的一些胚胎型MHC。因此，快肌MHC至少在妊娠17天时就已存在于次级和三级肌管中。