一种用于单克隆抗体高效放射性碘化的简易远程系统。
A simple remote system for the high-level radioiodination of monoclonal antibodies.
作者信息
Weadock K S, Anderson L L, Kassis A I
出版信息
J Nucl Med Allied Sci. 1989 Jan-Mar;33(1):37-41.
We have developed a system for the remote control radiolabeling of monoclonal antibodies (MoAb). The system is simple, inexpensive to assemble, and completely disposable. Reagents are pneumatically manipulated into vials containing Iodogen and an anion exchange resin, wherein iodination and separation of bound from free iodine occur, respectively, and sterilized (by filter) into a final vial. Radiolabeling yields of 38.9 +/- 5.5% were consistently produced with 99.3 +/- 0.2% of the isotope bound to the antibody. These results were not significantly different from those obtained in manual iodinations. However, the radiation dose to the hands of the laboratory worker in the remote mode was reduced at least ninefold. The system is presently being introduced for 300 to 400 mCi 131I iodinations of the anti-ovarian cancer MoAb OC 125 in a Phase I radioimmunotherapy trial.
我们开发了一种用于单克隆抗体（MoAb）远程控制放射性标记的系统。该系统简单，组装成本低，且完全可一次性使用。试剂通过气动方式被操作到含有碘甘醚和阴离子交换树脂的小瓶中，在其中分别进行碘化以及结合碘与游离碘的分离，然后（通过过滤）灭菌后进入最终的小瓶。放射性标记产率始终为38.9±5.5%，99.3±0.2%的同位素与抗体结合。这些结果与手动碘化法得到的结果无显著差异。然而，在远程模式下实验室工作人员手部所接受的辐射剂量至少降低了九倍。目前该系统正被引入用于一项I期放射免疫治疗试验中对治疗卵巢癌的单克隆抗体OC 125进行300至400毫居里131I的碘化。
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