Haisma H J, Hilgers J, Zurawski V R
J Nucl Med. 1986 Dec;27(12):1890-5.
We have developed a convenient system that can be used to iodinate monoclonal antibodies for diagnosis or therapy. A vial, previously coated with 1,3,4,6-tetrachloro-3a, 6a-diphenyl glycouril (iodogen), is used as a reaction vessel. Iodination and separation of bound and free iodide, using AG1-X8 ion exchange resin, are both accomplished in this vial. We found 90 +/- 4% of the iodide which was added was incorporated, respectively, into each of four different monoclonal antibodies evaluated. Approximately 90% of labeled antibody was recovered in each case. The monoclonal antibody OC125 was labeled to specific activities up to 25 mCi/mg. Immunoreactivities of 82 +/- 2% using 125I and 66 +/- 5% using 131I were achieved. As the radioiodination is done in one sealed vial and takes less than 15 min, this procedure is safe and can be performed in any nuclear medicine laboratory. The final product, which is sterile and apyrogenic, is suitable for diagnostic and radiotherapeutic applications.
我们开发了一种便捷的系统，可用于对单克隆抗体进行碘化，以用于诊断或治疗。一个预先涂有1,3,4,6 - 四氯 - 3a,6a - 二苯基甘脲（碘甘脲）的小瓶用作反应容器。使用AG1 - X8离子交换树脂进行碘化以及结合碘和游离碘的分离，均在该小瓶中完成。我们发现，添加的碘化物中分别有90±4%被掺入到所评估的四种不同单克隆抗体中。在每种情况下，大约90%的标记抗体被回收。单克隆抗体OC125的标记比活度高达25 mCi/mg。使用¹²⁵I时免疫反应性达到82±2%，使用¹³¹I时达到66±5%。由于放射性碘化在一个密封小瓶中进行且耗时不到15分钟，该程序安全且可在任何核医学实验室进行。最终产品无菌且无热原，适用于诊断和放射治疗应用。
