Simões Ana Leda Bertoncini, Figueira Rebeca Lopes, Gonçalves Frances Lilian Lanhellas, Mitidiero Luís Felipe Tsuyoshi, Silva Orlando Castro E, Peiró José Luis, Sbragia Lourenço
Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Divisão de Pediatria, Ribeirão Preto, SP/Brazil.
Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Cirurgia de Transplante, Ribeirão Preto, SP/Brazil.
Clinics (Sao Paulo). 2016 Jul;71(7):412-9. doi: 10.6061/clinics/2016(07)10.
Necrotizing enterocolitis is a severe multifactorial intestinal disorder that primarily affects preterm newborns, causing 20-40% mortality and morbidity. Intestinal fatty acid-binding protein has been reported to be a biomarker for the detection of intestinal injuries. Our aim was to assess intestinal tissue injury and the molecular expression of intestinal fatty acid-binding protein over time in a necrotizing enterocolitis model.
A total of 144 Newborn rats were divided into two groups: 1) Control, which received breastfeeding (n=72) and 2) Necrotizing Enterocolitis, which received formula feeding and underwent hypoxia and hypothermia (n=72). A total of six time points of ischemia (2 times a day for 3 days; 12 pups for each time point) were examined. Samples were collected for analysis of body weight, morphological and histological characteristics, intestinal weight, intestinal weight/body weight ratio, injury grade, and intestinal fatty acid-binding protein levels.
Body and intestinal weights were lower in the Necrotizing Enterocolitis group than in the Control group (p<0.005 and p<0.0005, respectively). The intestinal weight/body weight ratio was higher in the Necrotizing Enterocolitis group than in the Control group (p<0.005) only at the sixth ischemia time point. The Necrotizing Enterocolitis group displayed higher expression of intestinal fatty acid-binding protein (p<0.0005) and showed greater tissue damage than the Control group.
Intestinal fatty acid-binding protein was an efficient marker of ischemic injury to the intestine and a good correlation was demonstrated between the time of ischemic injury and the grade of intestinal injury.
坏死性小肠结肠炎是一种严重的多因素肠道疾病，主要影响早产新生儿，导致20%-40%的死亡率和发病率。据报道，肠脂肪酸结合蛋白是检测肠道损伤的生物标志物。我们的目的是在坏死性小肠结肠炎模型中评估肠道组织损伤以及肠脂肪酸结合蛋白随时间的分子表达。
总共144只新生大鼠被分为两组：1）对照组，接受母乳喂养（n=72）；2）坏死性小肠结肠炎组，接受配方奶喂养并经历缺氧和低温（n=72）。总共检查了六个缺血时间点（每天2次，共3天；每个时间点12只幼崽）。收集样本用于分析体重、形态和组织学特征、肠道重量、肠道重量/体重比、损伤等级和肠脂肪酸结合蛋白水平。
坏死性小肠结肠炎组的体重和肠道重量均低于对照组（分别为p<0.005和p<0.0005）。仅在第六个缺血时间点，坏死性小肠结肠炎组的肠道重量/体重比高于对照组（p<0.005）。坏死性小肠结肠炎组显示出更高的肠脂肪酸结合蛋白表达（p<0.0005），并且比对照组表现出更大的组织损伤。
肠脂肪酸结合蛋白是肠道缺血损伤的有效标志物，并且缺血损伤时间与肠道损伤等级之间存在良好的相关性。
