Wang Huiqiang, Rong Qinfeng, Ma Zhanfang
Department of Chemistry, Capital Normal University, Beijing, 100048, China.
Sci Rep. 2016 Jul 28;6:30623. doi: 10.1038/srep30623.
Polyhydroquinone-graphene composite as a new redox species was synthesized simply by a microwave-assisted one-pot method through oxidative polymerization of hydroquinone by graphene oxide, which exhibited excellent electrochemical redox activity at 0.124 V and can remarkably promote electron transfer. The as-prepared composite was used as immunosensing substrate in a label-free electrochemical immunosensor for the detection of cytokeratins antigen 21-1, a kind of biomarker of lung cancer. The proposed immunosensor showed wide liner range from 10 pg mL(-1) to 200 ng mL(-1) with a detection limit 2.3 pg mL(-1), and displayed a good stability and selectivity. In addition, this method has been used for the analysis of human serum sample, and the detection results showed good consistence with those of ELISA. The present substrate can be easily extended to other polymer-based nanocomposites.
通过氧化石墨烯对苯二酚的氧化聚合反应，采用微波辅助一锅法简单合成了聚对苯二酚-石墨烯复合材料，该复合材料在0.124 V时表现出优异的电化学氧化还原活性，能显著促进电子转移。所制备的复合材料用作无标记电化学免疫传感器的免疫传感底物，用于检测细胞角蛋白抗原21-1，一种肺癌生物标志物。所提出的免疫传感器线性范围宽，从10 pg mL(-1)到200 ng mL(-1)，检测限为2.3 pg mL(-1)，具有良好的稳定性和选择性。此外，该方法已用于人血清样本分析，检测结果与酶联免疫吸附测定法的结果具有良好的一致性。本底物可轻松扩展到其他基于聚合物的纳米复合材料。
