通过空斑试验和免疫染色试验对呼吸道合胞病毒感染性颗粒进行定量分析。
Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay.
作者信息
Jorquera Patricia A, Tripp Ralph A
机构信息
Department of Infectious Diseases, College of Veterinary Medicine, Animal Health Research Center, University of Georgia, 111 Carlton Street, Athens, GA, 30602, USA.
出版信息
Methods Mol Biol. 2016;1442:33-40. doi: 10.1007/978-1-4939-3687-8_3.
PMID:27464685
Abstract
One of the most commonly used approaches for determining the quantity of infectious RSV particles in a given sample is the plaque assay. RSV infectious particles can be quantified by various direct and indirect methods. Here, we explain two simple methods for RSV titration: plaque assay and immunostaining assay.
摘要
确定给定样本中传染性呼吸道合胞病毒（RSV）颗粒数量最常用的方法之一是蚀斑测定法。RSV传染性颗粒可以通过各种直接和间接方法进行定量。在此，我们解释两种简单的RSV滴定方法：蚀斑测定法和免疫染色测定法。
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