Bergeron Harrison C, Tripp Ralph A
Department of Infectious Diseases, University of Georgia, Athens, GA, USA.
Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Methods Mol Biol. 2025;2948:19-27. doi: 10.1007/978-1-0716-4666-3_2.
A useful and reproducible method to determine infectious respiratory syncytial virus (RSV) is to quantify virus plaques using immunostaining of infected Vero E6 cells. Immunostaining, in lieu of counterstaining, allows for accurate detection and quantification of RSV-specific plaques, avoiding spurious and nonspecific results. This method is important because RSV plaque morphology can be atypical in susceptible cell lines and often varies because it is a function of virus replication, cytopathic effect, and viral fitness. This protocol describes determining RSV titers using an immunostaining plaque assay on Vero E6 cells.
一种用于确定传染性呼吸道合胞病毒（RSV）的实用且可重复的方法是通过对感染的Vero E6细胞进行免疫染色来定量病毒蚀斑。使用免疫染色代替复染，能够准确检测和定量RSV特异性蚀斑，避免出现虚假和非特异性结果。该方法很重要，因为RSV蚀斑形态在易感细胞系中可能不典型，并且常常因病毒复制、细胞病变效应和病毒适应性而有所不同。本方案描述了使用对Vero E6细胞进行免疫染色蚀斑测定来确定RSV滴度的方法。
