Hanks C T, Diehl M L, Craig R G, Makinen P K, Pashley D H
Department of Oral Medicine, University of Michigan, Ann Arbor 48109-1078.
J Oral Pathol Med. 1989 Feb;18(2):97-107. doi: 10.1111/j.1600-0714.1989.tb00744.x.
Phenol was found to be an acceptable positive control substance for cytotoxicity assays in a new device, the "in vitro pulp chamber" (IVPC). Various concentrations of phenol were used to study its biologic effects on Balb/c 3T3 cells as well as its diffusion characteristics through various thicknesses of dentin disks. The cells showed a linear cytotoxic response to direct contact with phenol between the concentrations of 5 x 10(-4) and 5 x 10(-2) M. When a dentin barrier was used in the IVPC, phenol concentrations in the lower chamber were reduced below that in the reservoir and were inversely proportional to the thickness of the dentin. In the IVPC with 0.5 mm dentin disks, Balb/c 3T3 cells growing on the bottom of the dentin disks still maintained new protein synthesis at 60% of control levels when the phenol concentration in the reservoir was 0.05 M. When in direct contact with the cells this phenol concentration depressed protein synthesis to basal levels.
在一种新型装置“体外牙髓腔”（IVPC）中，苯酚被发现是细胞毒性检测可接受的阳性对照物质。使用了不同浓度的苯酚来研究其对Balb/c 3T3细胞的生物学效应，以及其通过不同厚度牙本质盘的扩散特性。在5×10⁻⁴至5×10⁻² M的浓度范围内，细胞与苯酚直接接触时呈现出线性细胞毒性反应。当在IVPC中使用牙本质屏障时，下腔室中的苯酚浓度低于储存器中的浓度，且与牙本质厚度成反比。在具有0.5毫米牙本质盘的IVPC中，当储存器中的苯酚浓度为0.05 M时，生长在牙本质盘底部的Balb/c 3T3细胞仍能维持新蛋白质合成，达到对照水平的60%。当该苯酚浓度与细胞直接接触时，蛋白质合成降至基础水平。
