Jung Eun Young, Park Yooheon, Woo Moon Jea, Lee Sang Hun, Suh Hyung Joo
Department of Home Economic Education, Jeonju University, Jeonju, 55069, Republic of Korea.
Institute of Biotechnology and Medical Converged Science, Dongguk University, Goyang, 10326, Republic of Korea.
Chin J Integr Med. 2016 Jul 28. doi: 10.1007/s11655-016-2598-7.
To assess the hematopoietic effects of fermented deer antler extract using a dietinduced anemic animal model to facilitate the utilization of fermented deer antler extract and its derived products.
Thirty 3-week-old female Sprague-Dawley rats were treated for 5 weeks. The rats were randomly divided into 6 groups and treated as follows: control, saline; NFA200, non-fermented deer antler extract 200 mg/kg; NFA500, non-fermented deer antler extract 500 mg/kg; FAB200, fermented deer antler extract 200 mg/kg; FAB500, fermented deer antler extract 500 mg/kg; and PC, heme iron 0.2 mg/kg. Blood parameters, iron content in the liver and spleen, hepatic δ-aminolevulinic acid dehydrogenase (ALAD) activity and divalent metal transporter 1 (DMT1) mRNA expression were analyzed.
No detectable significant differences were observed in blood parameters among groups. The decrease in the hepatic ALAD activity in anemic rats was significantly improved by fermented deer antler extract supplementation (P<0.05); however, non-fermented deer antler extract supplementation did not result in a significant improvement (P>0.05). The hepatic DMT1 mRNA expression level was increased significantly by supplementation with both the fermented deer antler extract and the non-fermented deer antler extract in a dose-dependent manner compared with nontreatment in anemic rats (P<0.05).
The hematopoietic activity induced by deer antler extract in dietinduced anemic rats might be increased through the fermentation process.
利用饮食诱导的贫血动物模型评估发酵鹿茸提取物的造血作用，以促进发酵鹿茸提取物及其衍生产品的利用。
对30只3周龄雌性Sprague-Dawley大鼠进行为期5周的治疗。将大鼠随机分为6组并进行如下处理：对照组，生理盐水；NFA200组，非发酵鹿茸提取物200mg/kg；NFA500组，非发酵鹿茸提取物500mg/kg；FAB200组，发酵鹿茸提取物200mg/kg；FAB500组，发酵鹿茸提取物500mg/kg；PC组，血红素铁0.2mg/kg。分析血液参数、肝脏和脾脏中的铁含量、肝脏δ-氨基乙酰丙酸脱氢酶（ALAD）活性以及二价金属转运蛋白1（DMT1）mRNA表达。
各组血液参数未观察到明显差异。补充发酵鹿茸提取物可显著改善贫血大鼠肝脏ALAD活性的降低（P<0.05）；然而，补充非发酵鹿茸提取物未产生显著改善（P>0.05）。与未治疗的贫血大鼠相比，补充发酵鹿茸提取物和非发酵鹿茸提取物均能显著提高肝脏DMT1 mRNA表达水平，且呈剂量依赖性（P<0.05）。
鹿茸提取物在饮食诱导的贫血大鼠中诱导的造血活性可能通过发酵过程增强。
