Park Yooheon, Choi Hyeon-Son, Lee Hyun-Sun, Suh Hyung Joo
Department of Food and Nutrition, Korea University, 5 Anam-dong, Sungbuk-gu, Seoul 136-713, Korea.
Department of Food Science and Technology, Seoul Women's University, Seoul 139-774, Korea.
Nutr Res Pract. 2015 Oct;9(5):451-8. doi: 10.4162/nrp.2015.9.5.451. Epub 2015 Sep 10.
BACKGROUND/OBJECTIVES: We examined the chemical composition and the effect of fermented deer antler on hematopoietic factors in bone marrow cells.
MATERIALS/METHODS: For the preparation of fermented deer antler extract (FAB), fermentation was carried out using Bacillus subtilis at 30℃ for 7 days. The hematopoietic effect of FAB was investigated hematopoietic factors in marrow cells.
The contents of total sugar, sulfated glycosaminoglycans, and uronic acid and the dry weight gradually increased with fermentation time. The sialic acid content (from 0.14 mg/mL to 0.54 mg/mL) was the highest on the 4th day of fermentation after which it decreased. The proliferating activity of bone marrow cells increased with fermentation times. The levels of various hematopoietic growth factors were determined to verify the beneficial effect of deer antler extract fermented by B. subtilis on hematopoiesis. FAB increased the number of stem cell factors and granulocyte colony-stimulating factor in bone marrow cells. In addition, FAB augmented the burst-forming unit erythroid and total colonies in splenocyte-conditioned medium compared with non-fermented antler extract (NFA). However, FAB did not affect the mRNA levels of erythropoietin, an important factor for erythropoiesis.
FAB, like NFA, did not directly affect hematopoiesis, but contributed to hematopoiesis by stimulating the production of hematopoietic factors.
背景/目的：我们研究了发酵鹿茸的化学成分及其对骨髓细胞造血因子的影响。
材料/方法：为制备发酵鹿茸提取物（FAB），使用枯草芽孢杆菌在30℃下进行7天发酵。通过骨髓细胞中的造血因子研究FAB的造血作用。
总糖、硫酸化糖胺聚糖、糖醛酸含量及干重随发酵时间逐渐增加。唾液酸含量（从0.14mg/mL至0.54mg/mL）在发酵第4天最高，之后下降。骨髓细胞的增殖活性随发酵时间增加。测定各种造血生长因子水平以验证枯草芽孢杆菌发酵的鹿茸提取物对造血的有益作用。FAB增加了骨髓细胞中干细胞因子和粒细胞集落刺激因子的数量。此外，与未发酵鹿茸提取物（NFA）相比，FAB增加了脾细胞条件培养基中的红系爆式形成单位和总集落数。然而，FAB不影响红细胞生成的重要因子促红细胞生成素的mRNA水平。
FAB与NFA一样，不直接影响造血，但通过刺激造血因子的产生促进造血。
